ESI ion trap-HPLC combines the considerable selectivity and sensitivity of electrospray ionization (ESI) mass spectrometry with fine analytical separations that can serve the research purposes of organic and bioorganic chemists. The four named investigators at the Department of Chemistry at UC Davis manage research that encompasses analytical, synthetic organic and bioorganic chemistry which are critically dependent upon access to MS. In recent years, the evolution of individual faculty research programs has brought demands for analytical methods that have outpaced available instrumentation. In order to meet the needs of four major users and various minor users in the Department of Chemistry, UC Davis, we request an HPLC -ESI ion trap mass spectrometer which will accommodate a range of research experiments involving small molecules (MW <2,000) to small proteins (MW<20,000) and provide new capabilities in soft ionization techniques in line with analytical chromatography. The critical need and common research theme for each major user is a need for real-time MS measurements of time-dependent phenomena (kinetics, liability studies, or analysis of separation & isolation of natural products that cannot be effectively accommodated by submission to routine MS services. The four major users manage a total of 6 major NIH RO1 or PO1 grants. Carlito Lebrilla studies the structures of sulfated and non-sulfated branched oligosaccharides using ESI MS/MS spectrometry. Claude Meares characterizes the structure of peptides chemically conjugated to metal chelating groups. Three natural products chemistry programs will be supported. Tadeusz F. Molinski studies the isolation and structure elucidation of biologically active small molecule components of marine invertebrates and the consensus binding site of the Ca2+ channel agonist, bastadin-5 with the RyR1 channel. Ben Shen investigates the molecular genetics, enzymology and temporal aspects of modular polyketide biosynthesis. The proportion of proposed instrument time allocated to the four major users will be 80% with the balance of the time made available to identified minor users within the department of chemistry.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR014701-01
Application #
6052112
Study Section
Special Emphasis Panel (ZRG1-BMT (02))
Program Officer
Tingle, Marjorie
Project Start
2000-03-01
Project End
2001-02-28
Budget Start
2000-03-01
Budget End
2001-02-28
Support Year
1
Fiscal Year
2000
Total Cost
$210,821
Indirect Cost
Name
University of California Davis
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Gilkerson, Robert W; Schon, Eric A; Hernandez, Evelyn et al. (2008) Mitochondrial nucleoids maintain genetic autonomy but allow for functional complementation. J Cell Biol 181:1117-28
Rogers, Evan W; Molinski, Tadeusz F (2007) Asymmetric synthesis of diastereomeric diaminoheptanetetraols. A proposal for the configuration of (+)-zwittermicin a. Org Lett 9:437-40
Corneillie, Todd M; Whetstone, Paul A; Meares, Claude F (2006) Irreversibly binding anti-metal chelate antibodies: Artificial receptors for pretargeting. J Inorg Biochem 100:882-90
Rogers, Evan W; Molinski, Tadeusz F (2005) A cytotoxic carotenoid from the marine sponge Prianos osiros. J Nat Prod 68:450-2
Corneillie, Todd M; Lee, Kelvin C; Whetstone, Paul A et al. (2004) Irreversible engineering of the multielement-binding antibody 2D12.5 and its complementary ligands. Bioconjug Chem 15:1392-402
Whetstone, Paul A; Butlin, Nathaniel G; Corneillie, Todd M et al. (2004) Element-coded affinity tags for peptides and proteins. Bioconjug Chem 15:3-6
Masuno, Makoto N; Molinski, Tadeusz F (2003) Cationic reduction of bastadin-4 to bastadin-5. Preparation of 5-[2h]-bastadin-5 by site-specific isotopic labeling. J Nat Prod 66:112-4
MacMillan, John B; Ernst-Russell, Michael A; de Ropp, Jeffrey S et al. (2002) Lobocyclamides A-C, lipopeptides from a cryptic cyanobacterial mat containing Lyngbya confervoides. J Org Chem 67:8210-5