A laser scanning confocal microscope system is requested for the Neurological Sciences Institute (NSI) of Oregon Health Sciences University in support of numerous NIH peer-reviewed grants. The vendor selected is Zeiss, whose latest technology, the Laser Scanning Microscope-510 with Non Linear Optics (LSM-510 NLO), is a state-of- the-art instrument that allows simultaneous multi-photon and single- photon excitation of biological tissue for the highest-sensitivity subcellular imaging and analysis of anatomical structure and physiological processes. The NSI will provide substantial support in the way of establishing a central core-facility for the instrument, cost- sharing, training, and a life-time maintenance and service contract. A group of 5 """"""""major users"""""""" with expertise in immunohistochemistry, fluorescence microscopy, neuroanatomy, and in vitro neurophysiology will employ 75% of the instrument's time. The balance of the instrument's time will be occupied by 8 additional faculty of the NSI. Altogether, the instrument will be used in support of 19 active NIH peer- reviewed awards from 9 different NIH institutes. The NSI is a newly formed group of 22 senior investigators that have been recruited from academic centers throughout the world that is housed in a brand new (February, 2001), 66,000 square foot, state-of-the-art building on the West Campus of Oregon Health Science University. The addition of a Zeiss LSM-510 NLO will make available to the newly assembled group essential capabilities in functional microscopy of subcellular processes.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR016858-01
Application #
6440353
Study Section
Special Emphasis Panel (ZRG1-SSS-U (03))
Program Officer
Tingle, Marjorie
Project Start
2002-05-01
Project End
2003-04-30
Budget Start
2002-05-01
Budget End
2003-04-30
Support Year
1
Fiscal Year
2002
Total Cost
$500,000
Indirect Cost
Name
Oregon Health and Science University
Department
Type
Schools of Medicine
DUNS #
009584210
City
Portland
State
OR
Country
United States
Zip Code
97239
Hermes, Sam M; Andresen, Michael C; Aicher, Sue A (2016) Localization of TRPV1 and P2X3 in unmyelinated and myelinated vagal afferents in the rat. J Chem Neuroanat 72:1-7
Hegarty, Deborah M; Hermes, Sam M; Largent-Milnes, Tally M et al. (2014) Capsaicin-responsive corneal afferents do not contain TRPV1 at their central terminals in trigeminal nucleus caudalis in rats. J Chem Neuroanat 61-62:1-12
Aicher, Sue A; Hegarty, Deborah M; Hermes, Sam M (2014) Corneal pain activates a trigemino-parabrachial pathway in rats. Brain Res 1550:18-26
Hermes, Sam M; Colbert, James F; Aicher, Sue A (2014) Differential content of vesicular glutamate transporters in subsets of vagal afferents projecting to the nucleus tractus solitarii in the rat. J Comp Neurol 522:642-53
Suckow, Shelby K; Deichsel, Emily L; Ingram, Susan L et al. (2013) Columnar distribution of catecholaminergic neurons in the ventrolateral periaqueductal gray and their relationship to efferent pathways. Synapse 67:94-108
Wilson-Poe, A R; Morgan, M M; Aicher, S A et al. (2012) Distribution of CB1 cannabinoid receptors and their relationship with mu-opioid receptors in the rat periaqueductal gray. Neuroscience 213:191-200
Aicher, Sue A; Hermes, Sam M; Whittier, Kelsey L et al. (2012) Descending projections from the rostral ventromedial medulla (RVM) to trigeminal and spinal dorsal horns are morphologically and neurochemically distinct. J Chem Neuroanat 43:103-11
Hegarty, Deborah M; Tonsfeldt, Karen; Hermes, Sam M et al. (2010) Differential localization of vesicular glutamate transporters and peptides in corneal afferents to trigeminal nucleus caudalis. J Comp Neurol 518:3557-69
Macey, T A; Ingram, S L; Bobeck, E N et al. (2010) Opioid receptor internalization contributes to dermorphin-mediated antinociception. Neuroscience 168:543-50
Nakamura, Y; Nakamura, K; Morrison, S F (2009) Different populations of prostaglandin EP3 receptor-expressing preoptic neurons project to two fever-mediating sympathoexcitatory brain regions. Neuroscience 161:614-20

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