Abstract

This application is a request from investigators at the Wake Forest University School of Medicine to upgrade our mass spectrometer facility by adding a Q-TOF Ultima Hybrid mass spectrometer to our facility. The new mass spectrometer will give us better quality mass spectra. The new instrument will expand our ability to analyze complex lipids, proteins and metabolites. Our present instrument, a Quattro II tandem quadrupole mass spectrometer, was installed in 1995 and has served us well, but does not have the capabilities of more modern instruments. The new mass spectrometer will provide 1) a significant increase in detection sensitivity, 2) higher resolution, 3) greater accuracy of mass measurement, 4) increased reliability, and 5) compatibility with new generations of software. ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR017846-01
Application #
6580736
Study Section
Special Emphasis Panel (ZRG1-BECM (02))
Program Officer
Tingle, Marjorie
Project Start
2003-05-01
Project End
2005-04-30
Budget Start
2003-05-01
Budget End
2005-04-30
Support Year
1
Fiscal Year
2003
Total Cost
$496,695
Indirect Cost

  Institution

Name
Wake Forest University Health Sciences
Department
Biochemistry
Type
Schools of Medicine
DUNS #
937727907
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157

  Publications

Payne, William M; Svechkarev, Denis; Kyrychenko, Alexander et al. (2018) The role of hydrophobic modification on hyaluronic acid dynamics and self-assembly. Carbohydr Polym 182:132-141
Svechkarev, Denis; Kyrychenko, Alexander; Payne, William M et al. (2018) Probing the self-assembly dynamics and internal structure of amphiphilic hyaluronic acid conjugates by fluorescence spectroscopy and molecular dynamics simulations. Soft Matter 14:4762-4771
Svechkarev, Denis; Sadykov, Marat R; Bayles, Kenneth W et al. (2018) Ratiometric Fluorescent Sensor Array as a Versatile Tool for Bacterial Pathogen Identification and Analysis. ACS Sens 3:700-708
Qi, Bowen; Crawford, Ayrianne J; Wojtynek, Nicholas E et al. (2018) Indocyanine green loaded hyaluronan-derived nanoparticles for fluorescence-enhanced surgical imaging of pancreatic cancer. Nanomedicine 14:769-780
Bhattacharya, D; Svechkarev, D; Souchek, J J et al. (2017) Impact of structurally modifying hyaluronic acid on CD44 interaction. J Mater Chem B 5:8183-8192
Hill, Tanner K; Davis, Amanda L; Wheeler, Frances B et al. (2016) Development of a Self-Assembled Nanoparticle Formulation of Orlistat, Nano-ORL, with Increased Cytotoxicity against Human Tumor Cell Lines. Mol Pharm 13:720-8
Pollard, Ricquita D; Fulp, Brian; Sorci-Thomas, Mary G et al. (2016) High-Density Lipoprotein Biogenesis: Defining the Domains Involved in Human Apolipoprotein A-I Lipidation. Biochemistry 55:4971-81
Hill, Tanner K; Kelkar, Sneha S; Wojtynek, Nicholas E et al. (2016) Near Infrared Fluorescent Nanoparticles Derived from Hyaluronic Acid Improve Tumor Contrast for Image-Guided Surgery. Theranostics 6:2314-2328
Pollard, Ricquita D; Blesso, Christopher N; Zabalawi, Manal et al. (2015) Procollagen C-endopeptidase Enhancer Protein 2 (PCPE2) Reduces Atherosclerosis in Mice by Enhancing Scavenger Receptor Class B1 (SR-BI)-mediated High-density Lipoprotein (HDL)-Cholesteryl Ester Uptake. J Biol Chem 290:15496-511
Chadwick, Alexandra C; Holme, Rebecca L; Chen, Yiliang et al. (2015) Acrolein impairs the cholesterol transport functions of high density lipoproteins. PLoS One 10:e0123138

Showing the most recent 10 out of 19 publications