Abstract

We propose to purchase a 500 MHz solid-state NMR (SSNMR) spectrometer that will be used for biomedical research by the Biophysics, Chemistry, Dental and Medical communities at the University of Michigan. ? ? At the University of Michigan, researchers utilize SSNMR spectroscopy to obtain high-resolution information about the structure, dynamics, geometry and interactions of non-soluble and/or non-crystalline biological systems. Progress of these NIH-funded research projects is hampered due to lack of a sophisticated high field SSNMR spectrometer. Therefore, this purchase will significantly extend the applicability of SSNMR spectroscopy to research projects that are presently under study at our institution and nucleate new initiatives in interdisciplinary research. ? ? The 500 MHz spectrometer will be used to study the high-resolution structure of membrane-associated proteins, regulatory RNAs that are important targets for development of anti-HIV therapeutics, and polymorphic pharmaceutical drugs. In addition, investigation of bone materials and membrane interaction of peptide antibiotics and dendrimers will significantly benefit from the use of the spectrometer. ? ? All the major users of the instrument are supported by NIH R01 grants. ? ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR023597-01
Application #
7214442
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (30))
Program Officer
Tingle, Marjorie
Project Start
2007-01-15
Project End
2009-07-14
Budget Start
2007-01-15
Budget End
2009-07-14
Support Year
1
Fiscal Year
2007
Total Cost
$500,000
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Kang, Juhye; Lee, Shin Jung C; Nam, Jung Seung et al. (2017) An Iridium(III) Complex as a Photoactivatable Tool for Oxidation of Amyloidogenic Peptides with Subsequent Modulation of Peptide Aggregation. Chemistry 23:1645-1653
Ravula, Thirupathi; Barnaba, Carlo; Mahajan, Mukesh et al. (2017) Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b5. Chem Commun (Camb) 53:12798-12801
Barnaba, Carlo; Gentry, Katherine; Sumangala, Nirupama et al. (2017) The catalytic function of cytochrome P450 is entwined with its membrane-bound nature. F1000Res 6:662
Zhang, Rongchun; Damron, Joshua; Vosegaard, Thomas et al. (2015) A cross-polarization based rotating-frame separated-local-field NMR experiment under ultrafast MAS conditions. J Magn Reson 250:37-44
Sudheendra, U S; Dhople, Vishnu; Datta, Aritreyee et al. (2015) Membrane disruptive antimicrobial activities of human ?-defensin-3 analogs. Eur J Med Chem 91:91-9
Lee, Sanghyun; Zheng, Xueyun; Krishnamoorthy, Janarthanan et al. (2014) Rational design of a structural framework with potential use to develop chemical reagents that target and modulate multiple facets of Alzheimer's disease. J Am Chem Soc 136:299-310
Pandey, Manoj Kumar; Vivekanandan, Subramanian; Yamamoto, Kazutoshi et al. (2014) Proton-detected 2D radio frequency driven recoupling solid-state NMR studies on micelle-associated cytochrome-b(5). J Magn Reson 242:169-79
Mroue, Kamal H; Zhang, Rongchun; Zhu, Peizhi et al. (2014) Acceleration of natural-abundance solid-state MAS NMR measurements on bone by paramagnetic relaxation from gadolinium-DTPA. J Magn Reson 244:90-7
Brender, Jeffrey R; Heyl, Deborah L; Samisetti, Shyamprasad et al. (2013) Membrane disordering is not sufficient for membrane permeabilization by islet amyloid polypeptide: studies of IAPP(20-29) fragments. Phys Chem Chem Phys 15:8908-15
MacKinnon, Neil; Somashekar, Bagganahalli S; Tripathi, Pratima et al. (2013) MetaboID: a graphical user interface package for assignment of 1H NMR spectra of bodyfluids and tissues. J Magn Reson 226:93-9

Showing the most recent 10 out of 42 publications