The instrumentation requested will equip a Peptide Core Laboratory at the David Geffen School of Medicine at UCLA, one of the nation's premier biomedical research institutions. It will consist of a multiple peptide synthesizer for small and intermediate scale peptide production, a preparative HPLC unit with integrated on- line mass spec system, freeze drying apparatus, and a chemical safety hood. Peptides produced in this facility would be used to study: a) hepcidin, a recently discovered 23-residue peptide that is a master regulator of iron uptake and utilization in humans; b) retrocyclins, cyclic octadecapeptides that prevent infection by HIV-1, influenza and herpes viruses; c) beta-amyloid, a peptide whose properties are involved in the pathogenesis of Alzheimer's Disease; d) antioxidant peptides, such as D4F, with the potential to decrease the risk of atherosclerosis; e) immunoregulatory peptides, that could eventually improve the treatment of patients with autoimmune disorders, such as lupus; f) peptides that can block the receptor-mediated ability of anthrax toxins to enter and damage cells; and g) peptides that can functionalize novel nanocomposite materials (""""""""quantum dots""""""""), and promote their development as novel imaging and drug-delivery agents. ? Each of these projects depends on peptides that are not available """"""""off-the-shelf"""""""". Consequently, the studies are often impeded by the expense or difficulty of obtaining peptides that may be cyclic (retrocyclins, D4F), or have multiple disulfide bonds (retrocyclins and hepcidin). ? ? ?
| Huang, Fang-Ke; Zhang, Guoan; Lawlor, Kevin et al. (2017) Deep Coverage of Global Protein Expression and Phosphorylation in Breast Tumor Cell Lines Using TMT 10-plex Isobaric Labeling. J Proteome Res 16:1121-1132 |
