Abstract

A spinning disk confocal microscope equipped for long term live cell imaging is requested for the use of researchers at the Mount Sinai School of Medicine. Major users will use the instrument for studies intercellular transmission of human immunodeficiency virus (HIV), neuronal homeostasis, retinoic acid receptors and breast cancer, cellular nucleic acid uptake, and mechanisms of platelet formation. The instrument will be placed in and maintained by the Microscopy Shared Research Facility, an ongoing operation currently serving the research of more than one hundred Mount Sinai investigators. The facility's staff of two full time biomedical engineers will provide supervision and training for users of the requested instrument and will be responsible for its maintenance.

Public Health Relevance

The requested instrument will support research in virology, neurology, oncology, and hematology concerning human immunodeficiency virus (HIV), neuronal cell biology, retinoic acid receptors in breast cancer, nucleic acid uptake by cells, and the formation of blood platelets. The information generated could impact therapies for major human health problems including AIDS, breast cancer and neurodegenerative disorders and could have broad implications for a variety of gene therapy strategies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR024745-01A1
Application #
7595556
Study Section
Special Emphasis Panel (ZRG1-CB-D (30))
Program Officer
Levy, Abraham
Project Start
2009-04-01
Project End
2010-03-31
Budget Start
2009-04-01
Budget End
2010-03-31
Support Year
1
Fiscal Year
2009
Total Cost
$500,000
Indirect Cost

  Institution

Name
Icahn School of Medicine at Mount Sinai
Department
Neurosciences
Type
Schools of Medicine
DUNS #
078861598
City
New York
State
NY
Country
United States
Zip Code
10029

  Publications

Linde, Nina; Casanova-Acebes, Maria; Sosa, Maria Soledad et al. (2018) Macrophages orchestrate breast cancer early dissemination and metastasis. Nat Commun 9:21
Beyleveld, Grant; Chin, Daniel J; Moreno Del Olmo, Elena et al. (2018) Nucleolar Relocalization of RBM14 by Influenza A Virus NS1 Protein. mSphere 3:
Sánchez-Aparicio, Maria T; Feinman, Leighland J; García-Sastre, Adolfo et al. (2018) Paramyxovirus V Proteins Interact with the RIG-I/TRIM25 Regulatory Complex and Inhibit RIG-I Signaling. J Virol 92:
Sánchez-Aparicio, M T; Ayllón, J; Leo-Macias, A et al. (2017) Subcellular Localizations of RIG-I, TRIM25, and MAVS Complexes. J Virol 91:
Harper, Kathryn L; Sosa, Maria Soledad; Entenberg, David et al. (2016) Mechanism of early dissemination and metastasis in Her2+ mammary cancer. Nature :
Das, Suvendu; Ladell, Daniel S; Podgrabinska, Simona et al. (2010) Vascular endothelial growth factor-C induces lymphangitic carcinomatosis, an extremely aggressive form of lung metastases. Cancer Res 70:1814-24