We are requesting a versatile system to improve our ability and capacity for a variety of live cell imaging projects. The instrument would be located in the Light Microscopy Core Facility (LMCF), a large established core that serves over 550 users across the Duke Campus. The DeltaVision system we are requesting would bring vitally needed capabilities in terms of photobleaching/photoactivation experiments, FRET, rapid multichannel acquisition and z-sweep acquisition. The precision of the stage and optimized nature of the acquisition and 3D deconvolution would provide a state-of-the-art live cell imaging system for many users. The system will add vital bandwidth to our live cell imaging resources.
| Watson, Lewis J; Alexander, Kevin M; Mohan, Maradumane L et al. (2016) Phosphorylation of Src by phosphoinositide 3-kinase regulates beta-adrenergic receptor-mediated EGFR transactivation. Cell Signal 28:1580-92 |
| Esher, Shannon K; Ost, Kyla S; Kozubowski, Lukasz et al. (2016) Relative Contributions of Prenylation and Postprenylation Processing in Cryptococcus neoformans Pathogenesis. mSphere 1: |
| Nichols, Connie B; Ost, Kyla S; Grogan, Dayton P et al. (2015) Impact of Protein Palmitoylation on the Virulence Potential of Cryptococcus neoformans. Eukaryot Cell 14:626-35 |
| Jagannathan, Sujatha; Reid, David W; Cox, Amanda H et al. (2014) De novo translation initiation on membrane-bound ribosomes as a mechanism for localization of cytosolic protein mRNAs to the endoplasmic reticulum. RNA 20:1489-98 |
| Jagannathan, Sujatha; Hsu, Jack C-C; Reid, David W et al. (2014) Multifunctional roles for the protein translocation machinery in RNA anchoring to the endoplasmic reticulum. J Biol Chem 289:25907-24 |
