Abstract

This proposal requests a Thermo Fisher Scientific LTQ Orbitrap Velos mass spectrometer system that will be utilized in projects important to human health, for example: pathogenesis of neuro-AIDS, tuberculosis, malaria and other infectious diseases;diabetes and obesity, cancer;and neurotransmission. The requested instrument is the next generation Orbitrap instrument that has features making it ideally suited for the projects proposed. These projects emphasize studies of quantitative proteomics and posttranslational modifications. This instrument will be utilized in an established mass spectrometry resource laboratory with highly skilled staff scientists in a highly interactive environment. The instrument system will also benefit the recently funded Einstein NIDA Proteomics Center on development of NeuroAIDS and drug abuse.

Public Health Relevance

The requested instrument system will be utilized in projects important to human health, for example: pathogenesis of neuro-AIDS, tuberculosis, malaria and other infectious diseases;diabetes and obesity, cancer;and neurotransmission.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR029398-01
Application #
7838661
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (30))
Program Officer
Birken, Steven
Project Start
2010-06-10
Project End
2011-06-09
Budget Start
2010-06-10
Budget End
2011-06-09
Support Year
1
Fiscal Year
2010
Total Cost
$963,205
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Shenoy, N; Bhagat, T; Nieves, E et al. (2017) Upregulation of TET activity with ascorbic acid induces epigenetic modulation of lymphoma cells. Blood Cancer J 7:e587
Amorim Franco, Tathyana Mar; Favrot, Lorenza; Vergnolle, Olivia et al. (2017) Mechanism-Based Inhibition of the Mycobacterium tuberculosis Branched-Chain Aminotransferase by d- and l-Cycloserine. ACS Chem Biol 12:1235-1244
Lefurgy, S T; Malashkevich, V N; Aguilan, J T et al. (2016) Analysis of the Structure and Function of FOX-4 Cephamycinase. Antimicrob Agents Chemother 60:717-28
Hishimoto, Akitoyo; Nomaru, Hiroko; Ye, Kenny et al. (2016) Molecular Histochemistry Identifies Peptidomic Organization and Reorganization Along Striatal Projection Units. Biol Psychiatry 79:415-420
Poulin, Myles B; Du, Quan; Schramm, Vern L (2015) Chemoenzymatic Synthesis of (36)S Isotopologues of Methionine and S-Adenosyl-L-methionine. J Org Chem 80:5344-7
Carvallo, Loreto; Lopez, Lillie; Che, Fa-Yun et al. (2015) Buprenorphine decreases the CCL2-mediated chemotactic response of monocytes. J Immunol 194:3246-58
Silmon de Monerri, Natalie C; Yakubu, Rama R; Chen, Allan L et al. (2015) The Ubiquitin Proteome of Toxoplasma gondii Reveals Roles for Protein Ubiquitination in Cell-Cycle Transitions. Cell Host Microbe 18:621-33
Hirsch, Brett M; Burgos, Emmanuel S; Schramm, Vern L (2014) Transition-state analysis of 2-O-acetyl-ADP-ribose hydrolysis by human macrodomain 1. ACS Chem Biol 9:2255-62
Fleitz, Antoine; Nieves, Edward; Madrid-Aliste, Carlos et al. (2013) Enhanced detection of multiply phosphorylated peptides and identification of their sites of modification. Anal Chem 85:8566-76
Nardelli, Sheila C; Che, Fa-Yun; Silmon de Monerri, Natalie C et al. (2013) The histone code of Toxoplasma gondii comprises conserved and unique posttranslational modifications. MBio 4:e00922-13

Showing the most recent 10 out of 12 publications