Abstract

We have built a state of the art culture facility for toxin producing algae and cyanobacteria, purifying toxins, providing DNA, RNA and nucleotide probes for toxins, organisms, and specific genes. This Facility Core is a shared facility between the UM NIEHS MFBMS Center, the NSF NIEHS Center for Oceans and Human Health, and the ARCH program. It is funded in part by all programs, and provides similar services to ARCH and Center researchers. Dr. Rein has directed this Core since the inception of the ARCH program and will continue to do so. Initially, two projects, """"""""Human Metabolites of Algal Toxins"""""""" and """"""""Bacterial Metabolism of Brevetoxins"""""""" will utilize this core. Additional toxin related projects will be supported as needed.
Specific Aims for the Toxic Algae Core Facility are: Provide live cultures of toxin producing algae and cyanobacteria to ARCH scientists, Provide purified materials such as toxins, DNA or RNA to ARCH scientists, Provide a facility for the isolation and purification of toxins from algae and cyanobacteria, Provide toxin analysis / protocols and training on HPLC/ UV or HPLC/ fluorescence detection or enzyme inhibition assays or ELISA to ARCH scientists.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Minority Biomedical Research Support Thematic Project Grants (S11)
Project #
5S11ES011181-09
Application #
7929526
Study Section
Special Emphasis Panel (ZES1)
Project Start
Project End
Budget Start
2009-09-01
Budget End
2010-08-31
Support Year
9
Fiscal Year
2009
Total Cost
$116,604
Indirect Cost

  Institution

Name
Florida International University
Department
Type
DUNS #
071298814
City
Miami
State
FL
Country
United States
Zip Code
33199

  Publications

Jaja-Chimedza, Asha; Sanchez, Kristel; Gantar, Miroslav et al. (2017) Carotenoid glycosides from cyanobacteria are teratogenic in the zebrafish (Danio rerio) embryo model. Chemosphere 174:478-489
Sun, Pengfei; Leeson, Cristian; Zhi, Xiaoduo et al. (2016) Characterization of an epoxide hydrolase from the Florida red tide dinoflagellate, Karenia brevis. Phytochemistry 122:11-21
Stice, Szabina; Liu, Guangliang; Matulis, Shannon et al. (2016) Determination of multiple human arsenic metabolites employing high performance liquid chromatography inductively coupled plasma mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 1009-1010:55-65
Hu, Ligang; Cai, Yong; Jiang, Guibin (2016) Occurrence and speciation of polymeric chromium(III), monomeric chromium(III) and chromium(VI) in environmental samples. Chemosphere 156:14-20
Jaja-Chimedza, Asha; Saez, Christopher; Sanchez, Kristel et al. (2015) Identification of teratogenic polymethoxy-1-alkenes fromCylindrospermopsis raciborskii, and taxonomically diverse freshwater cyanobacteria and green algae. Harmful Algae 49:156-161
Walton, Katherine; Gantar, Miroslav; Gibbs, Patrick D L et al. (2014) Indole alkaloids from Fischerella inhibit vertebrate development in the zebrafish (Danio rerio) embryo model. Toxins (Basel) 6:3568-81
Pettay, D Tye; Lajeunesse, Todd C (2013) Long-range dispersal and high-latitude environments influence the population structure of a ""stress-tolerant"" dinoflagellate endosymbiont. PLoS One 8:e79208
Rivera-Reyna, Nidia; Hinojosa-Reyes, Laura; Guzman-Mar, Jorge Luis et al. (2013) Photocatalytical removal of inorganic and organic arsenic species from aqueous solution using zinc oxide semiconductor. Photochem Photobiol Sci 12:653-9
Hu, Ligang; Greer, Justin B; Solo-Gabriele, Helena et al. (2013) Arsenic toxicity in the human nerve cell line SK-N-SH in the presence of chromium and copper. Chemosphere 91:1082-7
Liu, Guangliang; Cai, Yong (2013) Studying arsenite-humic acid complexation using size exclusion chromatography-inductively coupled plasma mass spectrometry. J Hazard Mater 262:1223-9

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