The goal of the proposed Yale Biophysics Training Program (BTP) is provide pre-doctoral students with strong quantitative training that will allow them to perform research at the forefront of biomedical science. The research topics derive from the diverse interests of our 34 faculty mentors and span areas from the study of the quantum mechanics of photosynthesis, the physics of NMR pulse sequences, to the structural biology of macromolecular machines. Our student trainees benefit from access to world-class expertise in X-Ray crystallography, optical and magnetic spectroscopies, cryo-electron microscopy, computational chemistry, biochemistry, and physical chemistry. Furthermore, our students are trained in usage of state-of-the-art instrumentation. The organization of the BTP is structured to facilitate our mentor's rigorous training of students in biophysical research in a manner that fosters their creativity and intrepidness. At Yale and elsewhere the importance of biophysics on health related subjects is evident from the number of biophysical studies occurring across several academic departments. Consistent with this expansion of biophysics, our mentors come from many departments including Physics, Chemistry, Engineering, Molecular Biophysics and Biochemistry (MB&B), Genetics, Pharmacology, Immunology, and Biology. Students are admitted to the BTP either directly through the Chemistry Department or from MB&B through an umbrella Biochemistry, Quantitative Biology, Biophysics, and Structural Biology (BQBS) program. The BQBS track, by its association with medical school departments, has enabled the BTP access to an expanded number of students from which to draw upon. Likewise, the students that come to the BTP through Chemistry and MB&B have scientific backgrounds that range from physics, mathematics, engineering, and physical chemistry, to molecular biology, biochemistry and biology. In their first year, students satisfy curricular requirements and further enhance and broaden their backgrounds through elective coursework. Also during the first year, students acquaint themselves with research by performing three laboratory research rotations. Transitioning to full-time research, students take two qualifying exams designed to encourage independent thinking and research project design. Upon joining a mentor lab, dissertation research is performed with supervision from a thesis committee comprised of three faculty that are familiar with the student's research topic and meet regularly to discuss progress. This BTP aims to support students in year 2 and potentially in year 3, as long as the student remains active in biophysical research. Other training enhancements of this BTP that are designed to facilitate interactions and cohesion among the trainees include research in progress talks, BTP Director workshops, the outside invited speaker program, and the biophysical retreat. The training resources at Yale and the dedicated commitment to training by the mentors, facilitates the BTP goal to produce the next generation of scientists.

Public Health Relevance

This application proposes to train graduate students to become independent scientists in modern experimental and computational biophysics. This biophysical training is essential to understand the function of all biological macromolecules. This understanding is crucial to deciphering how aberrant biological processes cause disease and this understanding will benefit human health.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Institutional National Research Service Award (T32)
Project #
5T32GM008283-33
Application #
9967014
Study Section
NIGMS Initial Review Group (TWD)
Program Officer
Flicker, Paula F
Project Start
1988-09-30
Project End
2023-06-30
Budget Start
2020-07-01
Budget End
2021-06-30
Support Year
33
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Yale University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Buzovetsky, Olga; Tang, Chenxiang; Knecht, Kirsten M et al. (2018) The SAM domain of mouse SAMHD1 is critical for its activation and regulation. Nat Commun 9:411
Knecht, Kirsten M; Buzovetsky, Olga; Schneider, Constanze et al. (2018) The structural basis for cancer drug interactions with the catalytic and allosteric sites of SAMHD1. Proc Natl Acad Sci U S A 115:E10022-E10031
Kostylev, Mikhail A; Tuttle, Marcus D; Lee, Suho et al. (2018) Liquid and Hydrogel Phases of PrPC Linked to Conformation Shifts and Triggered by Alzheimer's Amyloid-? Oligomers. Mol Cell 72:426-443.e12
Mentes, Ahmet; Huehn, Andrew; Liu, Xueqi et al. (2018) High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing. Proc Natl Acad Sci U S A 115:1292-1297
Kumar, Nikit; Leonzino, Marianna; Hancock-Cerutti, William et al. (2018) VPS13A and VPS13C are lipid transport proteins differentially localized at ER contact sites. J Cell Biol 217:3625-3639
Sinclair, Julie K L; Walker, Allison S; Doerner, Amy E et al. (2018) Mechanism of Allosteric Coupling into and through the Plasma Membrane by EGFR. Cell Chem Biol 25:857-870.e7
Chen, Shuliang; Bonifati, Serena; Qin, Zhihua et al. (2018) SAMHD1 suppresses innate immune responses to viral infections and inflammatory stimuli by inhibiting the NF-?B and interferon pathways. Proc Natl Acad Sci U S A 115:E3798-E3807
Benedetti, Lorena; Barentine, Andrew E S; Messa, Mirko et al. (2018) Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci U S A 115:E2238-E2245
St Gelais, Corine; Kim, Sun Hee; Maksimova, Victoria V et al. (2018) A Cyclin-Binding Motif in Human SAMHD1 Is Required for Its HIV-1 Restriction, dNTPase Activity, Tetramer Formation, and Efficient Phosphorylation. J Virol 92:
Iwamoto, Daniel V; Huehn, Andrew; Simon, Bertrand et al. (2018) Structural basis of the filamin A actin-binding domain interaction with F-actin. Nat Struct Mol Biol 25:918-927

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