Abstract

This program will produce the HIV envelope proteins and the T4 glycoprotein from a variety of expression vectors designed to make the proteins in bacteria, yeast or mammalian cells. The program will function as a core to provide material to virtually all of the other programs. It will supply native gp120/gp41 and native T4 as well as mutated forms and fragments of these proteins to the other groups. A goal of this program is to develop even better systems to express gp120/gp41 and T4 then the ones presently available. This will include the development of SV40 packaging vectors and the creation of cell lines expressing high levels of the proteins of interest. The program will also study the regulation of HIV envelope gene expression and examine the effect of the transactivating factors TAT and ART on expression from various vectors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01AI025721-03
Application #
3814668
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
State University of New York at Buffalo
Department
Type
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260

  Publications

Dundr, M; Meier, U T; Lewis, N et al. (1997) A class of nonribosomal nucleolar components is located in chromosome periphery and in nucleolus-derived foci during anaphase and telophase. Chromosoma 105:407-17
Dundr, M; Leno, G H; Lewis, N et al. (1996) Location of the HIV-1 Rev protein during mitosis: inactivation of the nuclear export signal alters the pathway for postmitotic reentry into nucleoli. J Cell Sci 109 ( Pt 9):2239-51
Berkowitz, R D; Hammarskjold, M L; Helga-Maria, C et al. (1995) 5' regions of HIV-1 RNAs are not sufficient for encapsidation: implications for the HIV-1 packaging signal. Virology 212:718-23
Srinivasakumar, N; Hammarskjold, M L; Rekosh, D (1995) Characterization of deletion mutations in the capsid region of human immunodeficiency virus type 1 that affect particle formation and Gag-Pol precursor incorporation. J Virol 69:6106-14
Dundr, M; Leno, G H; Hammarskjold, M L et al. (1995) The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev protein. J Cell Sci 108 ( Pt 8):2811-23
Mak, J; Jiang, M; Wainberg, M A et al. (1994) Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles. J Virol 68:2065-72
Hammarskjold, M L; Li, H; Rekosh, D et al. (1994) Human immunodeficiency virus env expression becomes Rev-independent if the env region is not defined as an intron. J Virol 68:951-8
Bray, M; Prasad, S; Dubay, J W et al. (1994) A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent. Proc Natl Acad Sci U S A 91:1256-60
Smith, A J; Srinivasakumar, N; Hammarskjold, M L et al. (1993) Requirements for incorporation of Pr160gag-pol from human immunodeficiency virus type 1 into virus-like particles. J Virol 67:2266-75
Keefer, M C; Bonnez, W; Roberts Jr, N J et al. (1991) Human immunodeficiency virus (HIV-1) gp160-specific lymphocyte proliferative responses of mononuclear leukocytes from HIV-1 recombinant gp160 vaccine recipients. J Infect Dis 163:448-53

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