Abstract

Two fundamental virologic issues represent major hurdles in the development of effective antiretroviral therapy: quantitation of viral load and drug resistance.
The aims of this proposal are to address each of these two issues in the laboratory by applying newly developed methods to clinical material from subjects on ACTG study protocols. In the area of quantitation, we propose to develop and optimize assays for viral infectivity, for viral DNA and RNA by PCR and for viral RNA by the self sustained sequence reaction (3SR) methodology. These methods will be applied to specific components of the peripheral blood including plasma, CD4 lymphocytes, monocyte/macrophages and dendritic cells. The quantitation of viral load, and the relative amounts of integrated and unintegrated DNA, in these blood components will be correlated with disease stage and antiretroviral therapy. In the area of drug resistance, methods that are being developed and optimized to assay for drug susceptibility of isolates of HIV and for mutations characteristic of drug resistance will be systematically applied to ACTG protocols. Only with precise and relevant markers of viral endpoints can reliable determinations be made of the impact of antiretroviral intervention.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project--Cooperative Agreements (U01)
Project #
2U01AI027670-10
Application #
5205318
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Beliakova-Bethell, Nadejda; Hezareh, Marjan; Wong, Joseph K et al. (2017) Relative efficacy of T cell stimuli as inducers of productive HIV-1 replication in latently infected CD4 lymphocytes from patients on suppressive cART. Virology 508:127-133
Osorio, Georgina; Hoenigl, Martin; Quartarolo, Jennifer et al. (2017) Evaluation of opt-out inpatient HIV screening at an urban teaching hospital. AIDS Care 29:1014-1018
Dan, Jennifer M; Massanella, Marta; Smith, Davey M et al. (2016) Brief Report: Effect of CMV and HIV Transcription on CD57 and PD-1 T-Cell Expression During Suppressive ART. J Acquir Immune Defic Syndr 72:133-7
Innes, Steve; Abdullah, Kameelah L; Haubrich, Richard et al. (2016) High Prevalence of Dyslipidemia and Insulin Resistance in HIV-infected Prepubertal African Children on Antiretroviral Therapy. Pediatr Infect Dis J 35:e1-7
Vanpouille, Christophe; Introini, Andrea; Morris, Sheldon R et al. (2016) Distinct cytokine/chemokine network in semen and blood characterize different stages of HIV infection. AIDS 30:193-201
Gianella, Sara; Ginocchio, Christine C; Daar, Eric S et al. (2016) Genital Epstein Barr Virus is associated with higher prevalence and persistence of anal human papillomavirus in HIV-infected men on antiretroviral therapy. BMC Infect Dis 16:24
Blumenthal, Jill; Jain, Sonia; Krakower, Douglas et al. (2015) Knowledge is Power! Increased Provider Knowledge Scores Regarding Pre-exposure Prophylaxis (PrEP) are Associated with Higher Rates of PrEP Prescription and Future Intent to Prescribe PrEP. AIDS Behav 19:802-10
Krishnan, Supriya; Schouten, Jeffrey T; Atkinson, Benjamin et al. (2015) Changes in metabolic syndrome status after initiation of antiretroviral therapy. J Acquir Immune Defic Syndr 68:73-80
Massanella, Marta; Gianella, Sara; Schrier, Rachel et al. (2015) Methamphetamine Use in HIV-infected Individuals Affects T-cell Function and Viral Outcome during Suppressive Antiretroviral Therapy. Sci Rep 5:13179
Gianella, Sara; Smith, Davey M; Daar, Eric S et al. (2015) Genital Cytomegalovirus Replication Predicts Syphilis Acquisition among HIV-1 Infected Men Who Have Sex with Men. PLoS One 10:e0130410

Showing the most recent 10 out of 318 publications