Abstract

This core facility will test immune sera and purified immunoglobulins for the ability to block HIV replication in established T-cell lines and fresh human peripheral blood mononuclear cells (PBMC). This will involve maintenance in tissue culture of MOLT-4, CEM PBMCs and other cells permissive for HIV-1 replication, production, tittering, and storage of viral stocks, establishment of HIV-1 chronically infected T cells for syncytium formation, testing of sera and immunoglobulin preparations for neutralization of HIV-1 isolates in syncytium inhibition and micro- reverse transcriptase assays, as well as analysis, storage and transmission of data to Principal Investigators of the NCVDG. Over 23 different immunogen/adjuvant formulations will be tested in mice, rabbits, rhesus macaques and samples per year which will need to be tested in HIV neutralization assays.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01AI035351-04S1
Application #
6268153
Study Section
Project Start
1996-12-01
Project End
1999-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
4
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Duke University
Department
Type
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705