Laboratory CoreMichael A. O'Donnell, MD will serve as director of the Lab Core with 5% total effort. He will perform the following tasks as director: (1) identify and set up an inventory of specific supplies required by each project with appropriate time lines, (2) develop system for cataloguing and storing all clinical specimens associated with the core projects, (3) prepare standard operating protocols (in consultation with Dr. Luo) for all laboratory assays to be performed in the Lab Core, (4) develop quality control measures to assure assay and data reliability, (5) develop a set of computer data files for storing and archiving all data from experiments related to Lab Core, (5) work as liaison between central project PI (Kreder) and individual Project Leaders (self, Luo, Lutgendorf to assure smooth coordination of specimen disbursement and assay completion, (6) maintain Lab Core budget, (7) directly supervise Dr. Xiaohong Chen, research technician for the Lab Core.Yi Luo, MD, PhD will serve as technical consultant for the project (no salary). As such, he will primarily provide assistance in developing the standard operating protocols for each of the Lab Core assays as well as provide inpute on troubleshooting and quality control. Dr. Luo wil further cover for Dr. O'Donnell on occasions when Dr. O'Donnell is not immediately available.Xiaohong Chen, MD, MS will be the research technician for the Lab Core with 30% effort. Dr. Chen is a senior research associate with over 10 years direct laboratory experience with Dr. O'Donnell. She has extensive experience with specimen preparation, tissue culture, cytokine and chemokine ELISA assays, immunestimulation assays, flow cytometry, and microscopy. She will order all supplies; collect, catalog, process and store all clinical specimens; separate out peripheral blood mononuclear cells (PBMCs); extract DNA for shipment to MD Anderson (Dr. Xifeng Wu's lab) for immune response genotyping, perform most of the ELISA and BioPlex quantitative immunoassays, and set up the immune stimulation assays. She will enter all data into computer files, perform the first level quantitative analysis, and prepare reports for the project Pis using the Lab Core. She will meet with Dr. O'Donnell at least twice weekly to coordinate and plan out Lab Core efforts.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project--Cooperative Agreements (U01)
Project #
1U01DK082344-01
Application #
7571857
Study Section
Special Emphasis Panel (ZDK1-GRB-G (M1))
Project Start
2008-09-15
Project End
2013-06-30
Budget Start
2008-09-15
Budget End
2009-06-30
Support Year
1
Fiscal Year
2008
Total Cost
$85,148
Indirect Cost
Name
University of Iowa
Department
Type
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242
Sutcliffe, Siobhan; Jemielita, Thomas; Lai, H Henry et al. (2018) A Case-Crossover Study of Urological Chronic Pelvic Pain Syndrome Flare Triggers in the MAPP Research Network. J Urol 199:1245-1251
Kogan, Paul; Xu, Suming; Wang, Yaoqin et al. (2018) Sub-noxious Intravesical Lipopolysaccharide Triggers Bladder Inflammation and Symptom Onset in A Transgenic Autoimmune Cystitis Model: A MAPP Network Animal Study. Sci Rep 8:6573
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Naliboff, Bruce D; Stephens, Alisa J; Lai, H Henry et al. (2017) Clinical and Psychosocial Predictors of Urological Chronic Pelvic Pain Symptom Change in 1 Year: A Prospective Study from the MAPP Research Network. J Urol 198:848-857
Kutch, Jason J; Labus, Jennifer S; Harris, Richard E et al. (2017) Resting-state functional connectivity predicts longitudinal pain symptom change in urologic chronic pelvic pain syndrome: a MAPP network study. Pain 158:1069-1082
Kutch, Jason J; Ichesco, Eric; Hampson, Johnson P et al. (2017) Brain signature and functional impact of centralized pain: a multidisciplinary approach to the study of chronic pelvic pain (MAPP) network study. Pain 158:1979-1991
Lai, H Henry; Jemielita, Thomas; Sutcliffe, Siobhan et al. (2017) Characterization of Whole Body Pain in Urological Chronic Pelvic Pain Syndrome at Baseline: A MAPP Research Network Study. J Urol 198:622-631
Dagher, Adelle; Curatolo, Adam; Sachdev, Monisha et al. (2017) Identification of novel non-invasive biomarkers of urinary chronic pelvic pain syndrome: findings from the Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network. BJU Int 120:130-142
Schrepf, Andrew; O'Donnell, Michael A; Luo, Yi et al. (2016) Inflammation and Symptom Change in Interstitial Cystitis or Bladder Pain Syndrome: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Study. Urology 90:56-61

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