Abstract

Public Health Relevance

Lysine-specific histone methyltransferases and histone demethylases are critical enzymes in the epigenetic regulation of DNA replication and repair, as well as recombination and gene expression and repression. Mutations in these enzymes have been implicated in a number of diseases. These studies will provide the biological rationale and structural basis for drug development.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project--Cooperative Agreements (U01)
Project #
1U01GM094588-01
Application #
8153269
Study Section
Special Emphasis Panel (ZGM1-CBB-0 (BC))
Project Start
2010-09-30
Project End
2015-06-30
Budget Start
2010-09-30
Budget End
2011-06-30
Support Year
1
Fiscal Year
2010
Total Cost
$1,360,605
Indirect Cost

  Institution

Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637

  Publications

Bailey, Lucas J; Sheehy, Kimberly M; Dominik, Pawel K et al. (2018) Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination. J Mol Biol 430:337-347
Mukherjee, Somnath; Griffin, Dionne H; Horn, James R et al. (2018) Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins. J Biol Chem 293:2815-2828
Sun, Jian; Paduch, Marcin; Kim, Sang-Ah et al. (2018) Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2. Proc Natl Acad Sci U S A 115:10010-10015
Schaefer, Zachary P; Bailey, Lucas J; Kossiakoff, Anthony A (2016) A polar ring endows improved specificity to an antibody fragment. Protein Sci 25:1290-8
Dominik, Pawel K; Borowska, Marta T; Dalmas, Olivier et al. (2016) Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs. Structure 24:300-9
Galilee, Meytal; Britan-Rosich, Elena; Griner, Sarah L et al. (2016) The Preserved HTH-Docking Cleft of HIV-1 Integrase Is Functionally Critical. Structure 24:1936-1946
Stuwe, Tobias; Correia, Ana R; Lin, Daniel H et al. (2015) Nuclear pores. Architecture of the nuclear pore complex coat. Science 347:1148-52
Dominik, Pawel K; Kossiakoff, Anthony A (2015) Phage display selections for affinity reagents to membrane proteins in nanodiscs. Methods Enzymol 557:219-45
Stuwe, Tobias; Bley, Christopher J; Thierbach, Karsten et al. (2015) Architecture of the fungal nuclear pore inner ring complex. Science 350:56-64
Borowska, Marta T; Dominik, Pawel K; Anghel, S Andrei et al. (2015) A YidC-like Protein in the Archaeal Plasma Membrane. Structure 23:1715-1724

Showing the most recent 10 out of 29 publications