Abstract

The Sampling Core serves as the most important resource for the Center Grant. It is through the operation of this Core that the HLA-typed blood donors who fall into the age range specified, and who donate blood on a regular basis, are identified and flagged so that the buffy coats can be obtained. The PBMC that are generated from the buddy coats are then distributed to the various projects, technical components or pilot projects that may require them. The second source of samples from this Core is bronchial alveolar lavage (BAL) cells that will be obtained from compensated donors. Our collaborators, Drs. Elizabeth Jacobs and Randolph Lipchik, at the Medical College of Wisconsin, will obtain the BAL. The Core will be responsible for a generation of a sample database, for sample distribution and archiving unused portions of the samples for possible later studies. The Core will perform some routine analyses on the samples that include surface marker analysis and measurement of influenza antibody titers. The Core is under the direction of Dr. Jack Gorski.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI062627-05
Application #
7673946
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2008-09-01
Budget End
2009-08-31
Support Year
5
Fiscal Year
2008
Total Cost
$239,310
Indirect Cost

  Institution

Name
Bloodcenter of Wisconsin, Inc.
Department
Type
DUNS #
057163172
City
Milwaukee
State
WI
Country
United States
Zip Code
53233

  Publications

Alarcon Falconi, T M; Cruz, M S; Naumova, E N (2018) The shift in seasonality of legionellosis in the USA. Epidemiol Infect 146:1824-1833
Yassai, Maryam B; Demos, Wendy; Gorski, Jack (2017) Structural and Mechanistic Implications of Rearrangement Frequencies within Human TCRBV Genes. J Immunol 199:1142-1152
Gil, Anna; Yassai, Maryam B; Naumov, Yuri N et al. (2015) Narrowing of human influenza A virus-specific T cell receptor ? and ? repertoires with increasing age. J Virol 89:4102-16
Zhou, Vivian; Yassai, Maryam B; Regunathan, Jeyarani et al. (2013) The functional CD8 T cell memory recall repertoire responding to the influenza A M1(58-66) epitope is polyclonal and shows a complex clonotype distribution. Hum Immunol 74:809-17
Levy, H; Wang, X; Kaldunski, M et al. (2012) Transcriptional signatures as a disease-specific and predictive inflammatory biomarker for type 1 diabetes. Genes Immun 13:593-604
Petrova, Galina; Ferrante, Andrea; Gorski, Jack (2012) Cross-reactivity of T cells and its role in the immune system. Crit Rev Immunol 32:349-72
Petrova, Galina V; Gorski, Jack (2012) Cross-reactive responses to modified M1??-?? peptides by CD8? T cells that use noncanonical BV genes can describe unknown repertoires. Eur J Immunol 42:3001-8
Baumgartner, Christina K; Yagita, Hideo; Malherbe, Laurent P (2012) A TCR affinity threshold regulates memory CD4 T cell differentiation following vaccination. J Immunol 189:2309-17
Moorthy, M; Castronovo, D; Abraham, A et al. (2012) Deviations in influenza seasonality: odd coincidence or obscure consequence? Clin Microbiol Infect 18:955-62
Bonacci, Benedetta; Edwards, Brandon; Jia, Shuang et al. (2012) Requirements for growth and IL-10 expression of highly purified human T regulatory cells. J Clin Immunol 32:1118-28

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