Abstract

The Radiobiological Standardization Core will provide standardized radiobiology tests for all projects within the CMCR with respect to tissue culture assays, animal model assays in the mouse and Zebrafish to ensure uniformity, reproducibility, and statistical significance of all radiobiological tests. The Radiobiological Standardization Core will function to deliver three specific aims:
Aim 1 : Ensure standardized in vitro cell culture, DNA damage quantitation, and comparative analysis of radioprotective and radiation mitigating functions of all new products developed by the five projects within the CMCR.
Aim 2 : Deliver timely reports and provide rapid analysis of cell culture and animal responses to new drugs developed by the five projects.
Aim 3 : Determine whether the Zebrafish represents an accurate, rapid, and inexpensive alternative to mouse assays for screening new radiobiological protectors and mitigators. The Radiobiological Standardization Core has as a main goal: the relief of the five projects from the diluted effort of doing their own tissue culture and animal model analysis of new products. By standardization of in vitro and in vivo radiation biology testing in this core facility, the five projects should be able to focus on the critical task of interaction between chemistry and radiation biology for drug discovery and development.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI068021-02
Application #
7310466
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2006-09-01
Budget End
2007-08-31
Support Year
2
Fiscal Year
2006
Total Cost
$219,548
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Knickelbein, Kyle; Tong, Jingshan; Chen, Dongshi et al. (2018) Restoring PUMA induction overcomes KRAS-mediated resistance to anti-EGFR antibodies in colorectal cancer. Oncogene 37:4599-4610
Wei, Liang; Leibowitz, Brian J; Epperly, Michael et al. (2018) The GS-nitroxide JP4-039 improves intestinal barrier and stem cell recovery in irradiated mice. Sci Rep 8:2072
Christner, Susan; Guo, Jianxia; Parise, Robert A et al. (2018) Liquid chromatography-tandem mass spectrometric assay for the quantitation of the novel radiation protective agent and radiation mitigator JP4-039 in murine plasma. J Pharm Biomed Anal 150:169-175
Wang, Yi-Jun; Fletcher, Rochelle; Yu, Jian et al. (2018) Immunogenic effects of chemotherapy-induced tumor cell death. Genes Dis 5:194-203
Chen, Dongshi; Tong, Jingshan; Yang, Liheng et al. (2018) PUMA amplifies necroptosis signaling by activating cytosolic DNA sensors. Proc Natl Acad Sci U S A 115:3930-3935
Chen, Dongshi; Ni, Hong-Min; Wang, Lei et al. (2018) PUMA induction mediates acetaminophen-induced necrosis and liver injury. Hepatology :
Chao, Honglu; Anthonymuthu, Tamil S; Kenny, Elizabeth M et al. (2018) Disentangling oxidation/hydrolysis reactions of brain mitochondrial cardiolipins in pathogenesis of traumatic injury. JCI Insight 3:
Steinman, Justin; Epperly, Michael; Hou, Wen et al. (2018) Improved Total-Body Irradiation Survival by Delivery of Two Radiation Mitigators that Target Distinct Cell Death Pathways. Radiat Res 189:68-83
Lou, Wenjia; Ting, Hsiu-Chi; Reynolds, Christian A et al. (2018) Genetic re-engineering of polyunsaturated phospholipid profile of Saccharomyces cerevisiae identifies a novel role for Cld1 in mitigating the effects of cardiolipin peroxidation. Biochim Biophys Acta Mol Cell Biol Lipids 1863:1354-1368
Anthonymuthu, Tamil S; Kenny, Elizabeth M; Lamade, Andrew M et al. (2018) Oxidized phospholipid signaling in traumatic brain injury. Free Radic Biol Med 124:493-503

Showing the most recent 10 out of 203 publications