Abstract

The goal of Core 3 (Genotyping) is to provide the infrastructure and technical expertise required to carryout the genetic-based studies proposed in the ICEMR. Core 3 will take advantage ofthe instrument infrastructure and the seven years of experience and expertise established by the Johns Hopkins Malaria Research Institute. Core 3 will provide services for the ICEMR investigators to generate high-quality genotyping, genomics and expression data using microarray, PCR and sequencing platforms. The Core will provide genotyping analysis of mosquito samples using PCR and lllumina platforms. Parasite samples will be genotyped employing the Affymetrix GeneChip and real time PCR platforms. Samples from human participants will be analyzed for multiplicity of infection using a nested PCR-based approach. Core 3 will facilitate in the analysis, interpretation and the integration ofthe data into the ICEMR database.

Public Health Relevance

Core 3 will provide the instrumentation and technical expertise to carryout the genotyping and expression analyses required to define the contribution of parasite, vector and human genetics to the transmission dynamics in the southern African ICEMR study sites.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI089680-04
Application #
8503403
Study Section
Special Emphasis Panel (ZAI1-AWA-M)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
4
Fiscal Year
2013
Total Cost
$57,236
Indirect Cost
$28,931

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Zawada, Jacek W; Dahan-Moss, Yael L; Muleba, Mbanga et al. (2018) Molecular and physiological analysis of Anopheles funestus swarms in Nchelenge, Zambia. Malar J 17:49
Jones, Christine M; Lee, Yoosook; Kitchen, Andrew et al. (2018) Complete Anopheles funestus mitogenomes reveal an ancient history of mitochondrial lineages and their distribution in southern and central Africa. Sci Rep 8:9054
Pringle, Julia C; Carpi, Giovanna; Almagro-Garcia, Jacob et al. (2018) RTS,S/AS01 malaria vaccine mismatch observed among Plasmodium falciparum isolates from southern and central Africa and globally. Sci Rep 8:6622
Kanyangarara, Mufaro; Hamapumbu, Harry; Mamini, Edmore et al. (2018) Malaria knowledge and bed net use in three transmission settings in southern Africa. Malar J 17:41
Searle, Kelly M; Lubinda, Jailos; Hamapumbu, Harry et al. (2017) Characterizing and quantifying human movement patterns using GPS data loggers in an area approaching malaria elimination in rural southern Zambia. R Soc Open Sci 4:170046
Searle, Kelly M; Katowa, Ben; Kobayashi, Tamaki et al. (2017) Distinct parasite populations infect individuals identified through passive and active case detection in a region of declining malaria transmission in southern Zambia. Malar J 16:154
Gibson, Lauren E; Markwalter, Christine F; Kimmel, Danielle W et al. (2017) Plasmodium falciparum HRP2 ELISA for analysis of dried blood spot samples in rural Zambia. Malar J 16:350
Ippolito, Matthew M; Searle, Kelly M; Hamapumbu, Harry et al. (2017) House Structure Is Associated with Plasmodium falciparum Infection in a Low-Transmission Setting in Southern Zambia. Am J Trop Med Hyg 97:1561-1567
Sutcliffe, Catherine G; Searle, Kelly; Matakala, Hellen K et al. (2017) Measles and Rubella Seroprevalence Among HIV-infected and Uninfected Zambian Youth. Pediatr Infect Dis J 36:301-306
Das, Smita; Muleba, Mbanga; Stevenson, Jennifer C et al. (2017) Beyond the entomological inoculation rate: characterizing multiple blood feeding behavior and Plasmodium falciparum multiplicity of infection in Anopheles mosquitoes in northern Zambia. Parasit Vectors 10:45

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