Inthisrenewalapplication,theScientificCore(CoreB)willcontinuetobuilduponthestrengthsofthe HumanImmuneMonitoringCenter(HIMC)andtheassaysdevelopedduringtheinitialgrantperiod.The HIMCisanidealenvironmentforstandardization,technologydevelopment,anddataanalysis,andhasbuilt anarrayofhigh-throughputandhigh-contentassaysthatareunparalleledinasinglecenterandinstitution. Overtheinitialfundingperiod,theScientificCoreaddedtothismenuoftechnologieswithuniqueCyTOF panelsandcombinedTCRseq/geneexpressionassaysatthesingle-celllevel.Overtheproposednext fundingperiod,CoreBwillcontinuetoprovidestandardizedimmunologicalassaysacrossstudieswithinthe U19project,focusingonsingle-cellassaysofallergen-responsivecellsandglobalimmuneprofiling.Itwill alsocontinuetodevelopnovelapproachesandworkwiththeindividualprojectstominetheimmunological datagenerated.
The specificaims are: 1.Toprovidestandardizedimmunemonitoringassaysacrossprojects.Thesewillincludedetailed immunophenotypingofwholebloodandPBMCsbymasscytometry(CyTOF),includingabasophil-centric CyTOFpaneldevelopedinthelabofSeanBendall(Co-InvestigatorofCoreB).TheCorewillalsooffer62- plexLuminexcytokinearraystoassessserumandcellsupernatantsforTh1/Th2/Th17cytokinesandsingle- cellTCRseq+geneexpressionforallergen-specificTcells.Finally,theCorewillperformMultiplexedIon BeamImaging(MIBI)ofesophageal,gastricandduodenalbiopsyspecimens,inthelabofRobertAngelo (Co-InvestigatorofCoreB).Byusingthesamestandardizedassaysacrossallcohorts,wewillgreatly increasethepowertotesthypothesesrelatedtoanyofthereadoutsoftheseassays. 2.Todevelopnovelimmunemonitoringassayswithindividualprojects.TheCyTOF,MIBI,andTCRseq panelswillcontinuetobedevelopedbasedonnewknowledgegeneratedwithintheprojects,aswellaskey observationsmadeintheallergyfieldgenerally.Wealsoplantoofferanewimmunoassayplatform, SomaScanfromSomaLogic,for1300solubleproteinanalytes,asanadditionaldiscoverytool. 3.Tofacilitateminingofimmunemonitoringdata.UsingStanfordDataMiner(SDM),theCorewillwork withtheDataManagementandAnalysisCoreCtominedatausingbioinformaticstools.SDMwillprovidea structuredframeworkforextractinglaboratorydatalinkedtoclinicalanddemographicvariables,facilitating dataminingandreducingthepossibilityoferrors.
| Klein, Ofir; Roded, Amit; Hirschberg, Koret et al. (2018) Imaging FITC-dextran as a Reporter for Regulated Exocytosis. J Vis Exp : |
| Zhang, Wenming; Lin, Chunrong; Sampath, Vanitha et al. (2018) Impact of allergen immunotherapy in allergic asthma. Immunotherapy 10:579-593 |
| Sampath, Vanitha; Sindher, Sayantani B; Zhang, Wenming et al. (2018) New treatment directions in food allergy. Ann Allergy Asthma Immunol 120:254-262 |
| Sindher, Sayantani B; Long, Andrew; Acharya, Swati et al. (2018) The Use of Biomarkers to Predict Aero-Allergen and Food Immunotherapy Responses. Clin Rev Allergy Immunol 55:190-204 |
| Mukai, Kaori; Tsai, Mindy; Saito, Hirohisa et al. (2018) Mast cells as sources of cytokines, chemokines, and growth factors. Immunol Rev 282:121-150 |
| Keren, Leeat; Bosse, Marc; Marquez, Diana et al. (2018) A Structured Tumor-Immune Microenvironment in Triple Negative Breast Cancer Revealed by Multiplexed Ion Beam Imaging. Cell 174:1373-1387.e19 |
| Chinthrajah, R Sharon; Purington, Natasha; Andorf, Sandra et al. (2018) Development of a tool predicting severity of allergic reaction during peanut challenge. Ann Allergy Asthma Immunol 121:69-76.e2 |
| Andorf, Sandra; Purington, Natasha; Block, Whitney M et al. (2018) Anti-IgE treatment with oral immunotherapy in multifood allergic participants: a double-blind, randomised, controlled trial. Lancet Gastroenterol Hepatol 3:85-94 |
| Tsai, Cheng-Ting; Mukai, Kaori; Robinson, Peter V et al. (2018) Isotype-specific agglutination-PCR (ISAP): A sensitive and multiplex method for measuring allergen-specific IgE. J Allergy Clin Immunol 141:1901-1904.e15 |
| Gaudenzio, Nicolas; Marichal, Thomas; Galli, Stephen J et al. (2018) Genetic and Imaging Approaches Reveal Pro-Inflammatory and Immunoregulatory Roles of Mast Cells in Contact Hypersensitivity. Front Immunol 9:1275 |
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