Abstract

This core will provide two fundamental benefits to the various telomere/telomerase projects. First, it will conserve resources by preventing unnecessary duplication of routine assays that may need to be performed by several of the participating groups. Secondly, it will achieve greater consistency between experiments that arise from the investigations of the research groups. The assays provided by this core are as follows: * To isolate and assay the telomerase activity in S100 extracts and freeze-thaw extracts from cultured tumor cells, hybrid cell lines, and tumors taken directly from patients. * To test whether telomerase activity is inhibited by various agents that are generated from Program l, Program 2. Program 3, the Chemistry Core, and from outside collaborative sources (N.C.I.), using our established procedures or the rapid assays proposed in program l. * To evaluate the growth inhibitory activity of telomere/telomerase interactive agents. * To determine telomere length. * To determine the number of dicentric chromosomes in cells. * To assess the clonogenic survival of tumor cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19CA067760-02
Application #
5208064
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Nishioka, David; Marcell, Vanessa; Cunningham, Meghan et al. (2003) The use of early sea urchin embryos in anticancer drug testing. Methods Mol Med 85:265-76
Kerwin, Sean M; Chen, Grace; Kern, Jonathan T et al. (2002) Perylene diimide G-quadruplex DNA binding selectivity is mediated by ligand aggregation. Bioorg Med Chem Lett 12:447-50
Kern, Jonathan T; Kerwin, Sean M (2002) The aggregation and G-quadruplex DNA selectivity of charged 3,4,9,10-perylenetetracarboxylic acid diimides. Bioorg Med Chem Lett 12:3395-8
Kern, Jonathan T; Thomas, Pei Wang; Kerwin, Sean M (2002) The relationship between ligand aggregation and G-quadruplex DNA selectivity in a series of 3,4,9,10-perylenetetracarboxylic acid diimides. Biochemistry 41:11379-89
Sun, Daekyu (2002) Biotinylated primer for detecting telomerase activity without amplification. Methods Mol Biol 191:165-71
Shi, D F; Wheelhouse, R T; Sun, D et al. (2001) Quadruplex-interactive agents as telomerase inhibitors: synthesis of porphyrins and structure-activity relationship for the inhibition of telomerase. J Med Chem 44:4509-23
Duan, W; Rangan, A; Vankayalapati, H et al. (2001) Design and synthesis of fluoroquinophenoxazines that interact with human telomeric G-quadruplexes and their biological effects. Mol Cancer Ther 1:103-20
Sun, D; Hurley, L H (2001) Targeting telomeres and telomerase. Methods Enzymol 340:573-92
Izbicka, E; Barnes, L D; Robinson, A K et al. (2001) Alterations in DNA repair and telomere maintenance mechanism affect response to porphyrins in yeast. Anticancer Res 21:1899-903
Han, H; Langley, D R; Rangan, A et al. (2001) Selective interactions of cationic porphyrins with G-quadruplex structures. J Am Chem Soc 123:8902-13

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