Core Leader: Thorsten R. Mempel. The Intravital Microscopy Core (Core A) has been formulated based on a stated need of U19 investigators and is designed to draw on the considerable experience of its key personnel in multiphoton intravital microscopy (MP-IVM) techniques to provide expertise and specialized equipment and aid in the design and execution of in vivo imaging studies of exRNA- and EV-mediated communication of glioblastomata with their stromal environment and the surrounding, healthy brain tissue.
The Specific Aims ofthe Intravital Microscopy Core are as follows: 1. To provide expertise in the design of specific experiments utilizing multi-photon microscopy-based analysis of EV biogenesis by glioblastomata, their properties in the extracellular space and uptake by nonmalignant brain cells, as well as of functional exRNA- and EV-mediated intercellular communication between tumor cells and tumor stroma. 2. To provide access to the required instrumentation and to aid in the execution of MP-IVM experiments using mouse models of glioblastoma developed by the project Pis with assistance of the core as outlined in aim 1. 3. To provide computational resources forthe storage, processing, in-depth analysis, and interpretation of digital imaging data generated in aim 2.
Services to be provided by Core A will allow participating investigators to examine by direct in vivo visualization approaches and and through use of various functional reporter systems multiple aspects of the biology of exRNAs and of EVs that are inaccessible to other, conventional experimental approaches.
| Ricklefs, Franz L; Alayo, Quazim; Krenzlin, Harald et al. (2018) Immune evasion mediated by PD-L1 on glioblastoma-derived extracellular vesicles. Sci Adv 4:eaar2766 |
| Zhou, Shuang; Appleman, Vicky A; Rose, Christopher M et al. (2018) Chronic platelet-derived growth factor receptor signaling exerts control over initiation of protein translation in glioma. Life Sci Alliance 1:e201800029 |
| Reátegui, Eduardo; van der Vos, Kristan E; Lai, Charles P et al. (2018) Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles. Nat Commun 9:175 |
| Boussiotis, Vassiliki A; Charest, Alain (2018) Immunotherapies for malignant glioma. Oncogene 37:1121-1141 |
| Marangoni, Francesco; Zhang, Ruan; Mani, Vinidhra et al. (2018) Tumor Tolerance-Promoting Function of Regulatory T Cells Is Optimized by CD28, but Strictly Dependent on Calcineurin. J Immunol 200:3647-3661 |
| Gustafsson, Gabriel; Lööv, Camilla; Persson, Emma et al. (2018) Secretion and Uptake of ?-Synuclein Via Extracellular Vesicles in Cultured Cells. Cell Mol Neurobiol 38:1539-1550 |
| György, Bence; Lööv, Camilla; Zaborowski, Miko?aj P et al. (2018) CRISPR/Cas9 Mediated Disruption of the Swedish APP Allele as a Therapeutic Approach for Early-Onset Alzheimer's Disease. Mol Ther Nucleic Acids 11:429-440 |
| Shao, Huilin; Im, Hyungsoon; Castro, Cesar M et al. (2018) New Technologies for Analysis of Extracellular Vesicles. Chem Rev 118:1917-1950 |
| Maas, Sybren L N; Breakefield, Xandra O; Weaver, Alissa M (2017) Extracellular Vesicles: Unique Intercellular Delivery Vehicles. Trends Cell Biol 27:172-188 |
| Wu, Anthony Yan-Tang; Lai, Charles Pin-Kuang (2017) Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters. Methods Mol Biol 1660:255-265 |
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