The long-range objectives of the UC Davis MMPC Complications and Pathology Core are to provide detailed metabolic phenotyping of mice for the complications of diabetes and obesity. We will focus our efforts on comprehensively phenotyping macrovascular and microvascular complications of diabetes and obesity. Diabetic and obesity phenotyping complications will be accomplished through the coordinated distribution and focused analyses of mice by the leader and co-leader of the core, Drs. Rutledge and Griffey, respectively. In addition, investigators using the core will gain access to the academic portal at UC Davis for comprehensive analysis of macrovascular and microvascular complications of diabetes and obesity. Investigators participating in this core and who have essential and special capabilities to phenotype macrovascular and microvascular complications are Drs. Rutledge, Griffey, Villablanca, Huser, Jin, Chiamvimovat, Ferrara, Nolta, and Van de Water.We have developed a comprehensive list of standard cardiovascular phenotyping assays. Review ofthe current list of national MMPC assays reveals needs in some areas. The UC Davis Complications and Pathology Core will fill some of these unmet needs using novel or new state-of-the-art approaches. These standard and new state-of-the-art and novel assays will be integrated with the other cores to better understand adipocyte biology, fatty liver disease, and insulin resistance.UC Davis has existing capabilities in mouse cardiovascular anatomy, physiology, pathology, and micro imaging that are outstanding. We will capitalize upon these assets to provide sophisticated cardiovascular phenotyping to users of the MMPC. Our mission is to ensure that efficient and accurate standard and novel and new state-of-the-art assays of submitted mice are provided to users ofthe UC Davis MMPC.

Public Health Relevance

Mouse models of diabetes, diabetic complications, obesity and other related disorders have been invaluable for elucidating the disease potential, pathogenesis and treatment of these conditions in the human population. The Complications and Pathology Core will conduct procedures and analyses on mouse lines submitted to the MMPC-UCD in order to identify potentail mouse models of human disease for study.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
5U24DK092993-04
Application #
8708053
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
4
Fiscal Year
2014
Total Cost
$167,631
Indirect Cost
$44,373
Name
University of California Davis
Department
Type
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Majtan, Tomas; Jones Jr, Wendell; Krijt, Jakub et al. (2018) Enzyme Replacement Therapy Ameliorates Multiple Symptoms of Murine Homocystinuria. Mol Ther 26:834-844
Yokoyama, Amy S; Dunaway, Keith; Rutkowsky, Jennifer et al. (2018) Chronic consumption of a western diet modifies the DNA methylation profile in the frontal cortex of mice. Food Funct 9:1187-1198
Rutkowsky, Jennifer M; Lee, Linda L; Puchowicz, Michelle et al. (2018) Reduced cognitive function, increased blood-brain-barrier transport and inflammatory responses, and altered brain metabolites in LDLr -/-and C57BL/6 mice fed a western diet. PLoS One 13:e0191909
Cheng, Yingduan; Yuan, Quan; Vergnes, Laurent et al. (2018) KDM4B protects against obesity and metabolic dysfunction. Proc Natl Acad Sci U S A 115:E5566-E5575
Rozman, Jan; Rathkolb, Birgit; Oestereicher, Manuela A et al. (2018) Identification of genetic elements in metabolism by high-throughput mouse phenotyping. Nat Commun 9:288
Hsu, Ming-Fo; Bettaieb, Ahmed; Ito, Yoshihiro et al. (2017) Protein tyrosine phosphatase Shp2 deficiency in podocytes attenuates lipopolysaccharide-induced proteinuria. Sci Rep 7:461
McGavigan, Anne K; Garibay, Darline; Henseler, Zachariah M et al. (2017) TGR5 contributes to glucoregulatory improvements after vertical sleeve gastrectomy in mice. Gut 66:226-234
Ito, Yoshihiro; Hsu, Ming-Fo; Bettaieb, Ahmed et al. (2017) Protein tyrosine phosphatase 1B deficiency in podocytes mitigates hyperglycemia-induced renal injury. Metabolism 76:56-69
López-Yoldi, Miguel; Stanhope, Kimber L; Garaulet, Marta et al. (2017) Role of cardiotrophin-1 in the regulation of metabolic circadian rhythms and adipose core clock genes in mice and characterization of 24-h circulating CT-1 profiles in normal-weight and overweight/obese subjects. FASEB J 31:1639-1649
Jung, Chris J; Zhang, Junli; Trenchard, Elizabeth et al. (2017) Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein. Transgenic Res 26:263-277

Showing the most recent 10 out of 74 publications