The Analytical Core of the Michigan Regional Comprehensive Metabolomics Resource Core (MRC2) will provide analytical tools to investigators to permit structural identification and quantification of metabolites in biospecimens. As a part of the MRC2, the Core will expand its Instrumentation infrastructure and enhance its services to serve to external and internal investigators who wish to metabolically phenotype biospecimens. Core staff will also provide consultation and collaboration in design of studies in preclinical, translational and clinical. Where needed, the Core laboratory will assist in development of new analytical methods to meet the research objectives of the investigators. The Analytical Core will optimize efficiency and cost effectiveness by providing these services to investigators through a central laboratory. The Core provides standardized analysis of a wide variety of small molecule metabolites from cells, tissues, plasma, and urine and will work with investigators to develop new assays. The Core also provides high-throughput, nondirected metabolite analysis for wider profiling of the metabolome. Research efforts will focus on three areas of need: first, applying the unique sensitivity and specificity of mass spectrometry to detect and quantify low abundance molecules of interest in biomedical research;second, the development of innovative mass spectrometric techniques for the detection and structural biomolecules;and three, providing MS training for graduate students and postdoctoral fellows with an interest in metabolomics research. Finally, the Analytical Core will enhance the services provided by the other MRC2 Cores by providing the data pipeline for the Data and Information Technology Core and Statistics and Bioinformatics Core and assist the Promotion and Outreach Core in training activities. The Core will coordinate with other Regional Comprehensive Metabolomics Resource Cores (RCMRCs) to develop data standards, detailed standard operating procedures for metabolomic analysis and data sharing. By centralizing and standardizing procedures, a common set of analytical tools will lead to a unified understanding of molecular mechanisms involved in physiologic and pathophysiologic processes underlying disease states.

Public Health Relevance

Metabolomics is an emerging technology that is underutilized In biomedical research. The proposed Analytical Core will expand the infrastructure and provide researchers with access to advanced metabolomic and lipomic phenotyping services to investigate the pathophysiology in experimental models of disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
1U24DK097153-01
Application #
8535386
Study Section
Special Emphasis Panel (ZRG1-BST-J (50))
Project Start
Project End
Budget Start
2012-09-04
Budget End
2013-08-31
Support Year
1
Fiscal Year
2012
Total Cost
$1,343,647
Indirect Cost
$114,683
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Gok, Emre; Alghanem, Fares; Moon, Ruth et al. (2018) Development of an Ex-Situ Limb Perfusion System for a Rodent Model. ASAIO J :
Bria, Carmen R M; Afshinnia, Farsad; Skelly, Patrick W et al. (2018) Asymmetrical flow field-flow fractionation for improved characterization of human plasma lipoproteins. Anal Bioanal Chem :
Maile, Michael D; Standiford, Theodore J; Engoren, Milo C et al. (2018) Associations of the plasma lipidome with mortality in the acute respiratory distress syndrome: a longitudinal cohort study. Respir Res 19:60
Perng, Wei; Tang, Lu; Song, Peter X K et al. (2018) Metabolomic profiles and development of metabolic risk during the pubertal transition: a prospective study in the ELEMENT Project. Pediatr Res :
Zarrinpar, Amir; Chaix, Amandine; Xu, Zhenjiang Z et al. (2018) Antibiotic-induced microbiome depletion alters metabolic homeostasis by affecting gut signaling and colonic metabolism. Nat Commun 9:2872
Ward, Kristen M; Yeoman, Larisa; McHugh, Cora et al. (2018) Atypical Antipsychotic Exposure May Not Differentiate Metabolic Phenotypes of Patients with Schizophrenia. Pharmacotherapy 38:638-650
Jadoon, Adil; Mathew, Anna V; Byun, Jaeman et al. (2018) Gut Microbial Product Predicts Cardiovascular Risk in Chronic Kidney Disease Patients. Am J Nephrol 48:269-277
Patel, Anita; Yusta, Bernardo; Matthews, Dianne et al. (2018) GLP-2 receptor signaling controls circulating bile acid levels but not glucose homeostasis in Gcgr-/- mice and is dispensable for the metabolic benefits ensuing after vertical sleeve gastrectomy. Mol Metab 16:45-54
Puskarich, Michael A; Evans, Charles R; Karnovsky, Alla et al. (2018) Septic Shock Nonsurvivors Have Persistently Elevated Acylcarnitines Following Carnitine Supplementation. Shock 49:412-419
Schofield, Heather K; Zeller, Jörg; Espinoza, Carlos et al. (2018) Mutant p53R270H drives altered metabolism and increased invasion in pancreatic ductal adenocarcinoma. JCI Insight 3:

Showing the most recent 10 out of 139 publications