Abstract

(MANAGEMENT AND DISSEMINATION) The overall goal of this proposal is to continue our high throughput analysis of the human interactome. Our experimental pipeline is organized in a process that involves wet-lab steps, proteomic steps, informatics steps, and dissemination of the data. Thus, dissemination of the data is a central aspect of the proposal. In this section, we describe both our management structure and the mechanisms we will use to disseminate the interaction data to the community. In addition, we will also disseminate molecular clones that are being produced in this work through the Dana Farber/Harvard Cancer Center DNA Resource Core Facility.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Biotechnology Resource Cooperative Agreements (U41)
Project #
2U41HG006673-04
Application #
8933270
Study Section
Ethical, Legal, Social Implications Review Committee (GNOM)
Project Start
Project End
Budget Start
2015-09-01
Budget End
2016-06-30
Support Year
4
Fiscal Year
2015
Total Cost
$60,437
Indirect Cost
$24,781

  Institution

Name
Harvard Medical School
Department
Type
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Schweppe, Devin K; Huttlin, Edward L; Harper, J Wade et al. (2018) BioPlex Display: An Interactive Suite for Large-Scale AP-MS Protein-Protein Interaction Data. J Proteome Res 17:722-726
Gu, Xin; Orozco, Jose M; Saxton, Robert A et al. (2017) SAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway. Science 358:813-818
Paek, Jaeho; Kalocsay, Marian; Staus, Dean P et al. (2017) Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity Labeling. Cell 169:338-349.e11
Anikster, Yair; Haack, Tobias B; Vilboux, Thierry et al. (2017) Biallelic Mutations in DNAJC12 Cause Hyperphenylalaninemia, Dystonia, and Intellectual Disability. Am J Hum Genet 100:257-266
Huttlin, Edward L; Bruckner, Raphael J; Paulo, Joao A et al. (2017) Architecture of the human interactome defines protein communities and disease networks. Nature 545:505-509
Chick, Joel M; Munger, Steven C; Simecek, Petr et al. (2016) Defining the consequences of genetic variation on a proteome-wide scale. Nature 534:500-5
Yang, Wen; Nagasawa, Koji; Münch, Christian et al. (2016) Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization. Cell 167:985-1000.e21
Chantranupong, Lynne; Scaria, Sonia M; Saxton, Robert A et al. (2016) The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. Cell 165:153-164
Huttlin, Edward L; Ting, Lily; Bruckner, Raphael J et al. (2015) The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 162:425-440
McAlister, Graeme C; Nusinow, David P; Jedrychowski, Mark P et al. (2014) MultiNotch MS3 enables accurate, sensitive, and multiplexed detection of differential expression across cancer cell line proteomes. Anal Chem 86:7150-8

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