The goal of this project is to expand production of rhesus macaques of Indian origin that are specific pathogen free (SPF) for Cercopithecine herpesvirus I, Simian T Lymphotrophic Virus I (STLV), Type D retrovirus, Simian Immunodeficiency Virus (SIV) and Simian Foamy Virus (SFV). This expansion will be directed towards production of SPF animals for AIDS investigators and establishment of a long term SPF breeding colony of animals with known pedigrees and defined genotypes. The production of pedigreed Indian rhesus will include genetic characterization of family lines and the major histocompatibility complex (MHC).. The CTL response is a critical measure in the evaluation of candidate vaccines for SIV. In this respect, Indian rhesus with the MHC Class I allele Mamu-A*01 are important for current research protocols because the appropriate reagents exist to measure viral-specific CTL.. Colony pedigree data will be used to establish zygosity of offspring and facilitate selective breeding for animals positive for the Mamu-A*01 allele. Assisted reproductive technology will be used to rapidly expand numbers of Mamu-A*01 males to accelerate colony production. The SPF pedigreed Indian rhesus colony will also serve as a long term resource for rapid expansion of animals numbers with other desirable MHC Class I alleles through selective breeding and assisted reproductive technology.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Materials Resource Cooperative Agreements (U42)
Project #
5U42RR016023-02
Application #
6394861
Study Section
Special Emphasis Panel (ZRR1-CM-9 (02))
Program Officer
Watson, William T
Project Start
2000-09-30
Project End
2005-09-29
Budget Start
2001-09-30
Budget End
2002-09-29
Support Year
2
Fiscal Year
2001
Total Cost
$1,302,324
Indirect Cost
Name
University of California Davis
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618