The WWAMI RCE will develop a strong program of basic research, education, and training in biodefense and emerging infectious diseases with a focus on Gram-negative pathogens. The objectives are: 1. Development of a regional program supported by modem biotechnology platforms, including proteomics, genomics, and bioinformatics dedicated to studying the biology and pathogenesis of Gram-negative bacterial agents of relevance to biodefense and emerging infectious diseases. The WWAMI RCE will initially focus on Yersinia pestis (YP), Francisella tularensis (FT), and Burkholderia pseudomallei (BP). 2. Development of a regional program to understand in greater detail the pathophysiology of airway infection and inflammation by Gram-negative pathogens. Animal models of airway infection will be utilized, including non-human primates and rodents. 3. Development of a regional program that will study differences in human susceptibility to Gram-negative bacterial agents of relevance to biodefense and emerging infectious diseases. Initial studies will define variability in the response of normal humans of varying genetic backgrounds to LPS from YP, FT, and synthetic LPS derived compounds. 4. Translate information obtained into preclinical testing of vaccine candidates and therapeutics that can be useful in protection against Gram-negative agents of relevance to biodefense and emerging infectious diseases. The initial focus will be on the development of vaccines for YP and the use of synthetic LPS derived compounds as adjuvants and innate immune stimulators that broadly protect against a variety of infectious agents. 5. Utilize the extensive WWAMI regional program in education and training to recruit new investigators and educate trainees about the field of biodefense and emerging infectious diseases. 5. Development of a regional plan to interface with the regional public health network to utilize WWAMI RCE resources in a national emergency.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
1U54AI057141-01
Application #
6698620
Study Section
Special Emphasis Panel (ZAI1-KLW-M (M3))
Program Officer
Dombroski, Alicia J
Project Start
2003-09-04
Project End
2008-02-29
Budget Start
2003-09-04
Budget End
2004-02-29
Support Year
1
Fiscal Year
2003
Total Cost
$4,440,215
Indirect Cost
Name
University of Washington
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Hagar, Jon A; Edin, Matthew L; Lih, Fred B et al. (2017) Lipopolysaccharide Potentiates Insulin-Driven Hypoglycemic Shock. J Immunol 199:3634-3643
Hajjar, Adeline M; Ernst, Robert K; Yi, Jaehun et al. (2017) Expression level of human TLR4 rather than sequence is the key determinant of LPS responsiveness. PLoS One 12:e0186308
Jorgensen, Ine; Lopez, Joseph P; Laufer, Stefan A et al. (2016) IL-1?, IL-18, and eicosanoids promote neutrophil recruitment to pore-induced intracellular traps following pyroptosis. Eur J Immunol 46:2761-2766
Miller, Samuel I; Chaudhary, Anu (2016) A Cellular GWAS Approach to Define Human Variation in Cellular Pathways Important to Inflammation. Pathogens 5:
Fan, Vincent S; Gharib, Sina A; Martin, Thomas R et al. (2016) COPD disease severity and innate immune response to pathogen-associated molecular patterns. Int J Chron Obstruct Pulmon Dis 11:467-77
Jorgensen, Ine; Zhang, Yue; Krantz, Bryan A et al. (2016) Pyroptosis triggers pore-induced intracellular traps (PITs) that capture bacteria and lead to their clearance by efferocytosis. J Exp Med 213:2113-28
Hayden, Hillary S; Matamouros, Susana; Hager, Kyle R et al. (2016) Genomic Analysis of Salmonella enterica Serovar Typhimurium Characterizes Strain Diversity for Recent U.S. Salmonellosis Cases and Identifies Mutations Linked to Loss of Fitness under Nitrosative and Oxidative Stress. MBio 7:e00154
Chaudhary, Anu; Leite, Mara; Kulasekara, Bridget R et al. (2016) Human Diversity in a Cell Surface Receptor that Inhibits Autophagy. Curr Biol 26:1791-801
Majerczyk, Charlotte; Schneider, Emily; Greenberg, E Peter (2016) Quorum sensing control of Type VI secretion factors restricts the proliferation of quorum-sensing mutants. Elife 5:
Yen, Gloria S; Edgar, J Scott; Yoon, Sung Hwan et al. (2016) Polydimethylsiloxane microchannel coupled to surface acoustic wave nebulization mass spectrometry. Rapid Commun Mass Spectrom 30:1096-100

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