Magnetic resonance molecular imaging strategies have been explored by specifically targetingoncogenes such as HER-2 (c-erbB-2, Neu), bcl-2/bcl-xL, protein kinase A, and the transferrin receptorgene. The human transferrin receptor (hTfR) has been used as a molecular target to direct therapeuticagents to tumor cells and to shuttle drugs across the blood-brain barrier. Expression and regulation ofhTfR'receptors can be visualized by NMR imaging by studying the receptors with a sterically protectingiron-containing magnetic hTfR probe. A cationic immunoliposome system which covalently conjugatesthe single-chain antibody variable region fragment (scFv) against the hTfR has been used to improvep53 tumor suppressor gene therapy employed in the human breast cancer metastasis model. ThisscFv-immunolipdsome can systematically deliver the complexed gene to tumors in vivo. In comparisonto a whole antibody or transferrin molecule, scFv has a much smaller size, enabling it to penetrate moreeasily through solid tumors. In this study, we will utilize the TfR scFv-immunoliposome along with MRcontrast agents to improve contrast agent-tumor affinity and specificity. This will enhance MRdiagnostic imaging capabilities, particularly those needed for early detection.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
2U54CA091409-06A1
Application #
7250610
Study Section
Special Emphasis Panel (ZCA1-SRRB-Y (O1))
Project Start
2006-12-01
Project End
2011-08-31
Budget Start
2006-12-01
Budget End
2007-08-31
Support Year
6
Fiscal Year
2006
Total Cost
$81,062
Indirect Cost
Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Weber, Kari A; Heaphy, Christopher M; Joshu, Corinne E et al. (2018) Racial differences in maternal and umbilical cord blood leukocyte telomere length and their correlations. Cancer Causes Control 29:759-767
Weber, Kari A; Heaphy, Christopher M; Rohrmann, Sabine et al. (2016) Influence of In Utero Maternal and Neonate Factors on Cord Blood Leukocyte Telomere Length: Clues to the Racial Disparity in Prostate Cancer? Prostate Cancer 2016:3691650
Antony, Lizamma; van der Schoor, Freek; Dalrymple, Susan L et al. (2014) Androgen receptor (AR) suppresses normal human prostate epithelial cell proliferation via AR/?-catenin/TCF-4 complex inhibition of c-MYC transcription. Prostate 74:1118-31
Wilson-Frederick, Shondelle M; Thorpe Jr, Roland J; Bell, Caryn N et al. (2014) Examination of race disparities in physical inactivity among adults of similar social context. Ethn Dis 24:363-9
Dejea, Christine M; Wick, Elizabeth C; Hechenbleikner, Elizabeth M et al. (2014) Microbiota organization is a distinct feature of proximal colorectal cancers. Proc Natl Acad Sci U S A 111:18321-6
Rohrmann, Sabine; Platz, Elizabeth A (2014) To adjust or not in studies on racial differences in hormone concentrations? Depends on the question! J Clin Endocrinol Metab 99:407-8
Milam, Adam J; Bone, Lee R; Byron, M Justin et al. (2013) Cigarillo use among high-risk urban young adults. J Health Care Poor Underserved 24:1657-65
Martinez, Kathryn A; Pollack, Craig E; Phelan, Darcy F et al. (2013) Gender differences in correlates of colorectal cancer screening among Black Medicare beneficiaries in Baltimore. Cancer Epidemiol Biomarkers Prev 22:1037-42
Eichholzer, Monika; Platz, Elizabeth A; Bienstock, Jessica L et al. (2013) Racial variation in vitamin D cord blood concentration in white and black male neonates. Cancer Causes Control 24:91-8
Shinohara, Debika Biswal; Vaghasia, Ajay M; Yu, Shu-Han et al. (2013) A mouse model of chronic prostatic inflammation using a human prostate cancer-derived isolate of Propionibacterium acnes. Prostate 73:1007-15

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