Abstract

The Proteomics Core is focused on the application and continued improvement of cutting-edge proteomic technologies to support the identification and validation of nitrotyrosine and related post translational modifications of proteins as biomarkers of cigarette-smoke induced inflammatory/oxidative stress. The overall strategy involves utilizing continued advances in proteomics instrumentation, methodologies, and informatics tools to perform ultra-sensitive, high-throughput analysis of the large numbers of both mouse model and human clinical samples necessary to make biologically meaningful conclusions. These studies include both sensitive global biomarker discovery efforts as well as more focused data directed (multiplereaction- monitoring, MRM) validation based studies. Significant proteomic technological advances have been developed by the Smith group at PNNL which will be leveraged by this core in support of the research efforts.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54ES016015-04
Application #
8109316
Study Section
Special Emphasis Panel (ZES1)
Project Start
Project End
Budget Start
2010-07-06
Budget End
2012-05-31
Support Year
4
Fiscal Year
2010
Total Cost
$251,963
Indirect Cost

  Institution

Name
Battelle Pacific Northwest Laboratories
Department
Type
DUNS #
032987476
City
Richland
State
WA
Country
United States
Zip Code
99352

  Publications

Tanaka, Naoki; Takahashi, Shogo; Hu, Xiao et al. (2017) Growth arrest and DNA damage-inducible 45? protects against nonalcoholic steatohepatitis induced by methionine- and choline-deficient diet. Biochim Biophys Acta Mol Basis Dis 1863:3170-3182
Takahashi, Shogo; Fukami, Tatsuki; Masuo, Yusuke et al. (2016) Cyp2c70 is responsible for the species difference in bile acid metabolism between mice and humans. J Lipid Res 57:2130-2137
Song, Yang; Luo, Yanan; Zhu, Chengzhou et al. (2016) Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials. Biosens Bioelectron 76:195-212
Webb-Robertson, Bobbie-Jo M; Wiberg, Holli K; Matzke, Melissa M et al. (2015) Review, evaluation, and discussion of the challenges of missing value imputation for mass spectrometry-based label-free global proteomics. J Proteome Res 14:1993-2001
Zhu, Chengzhou; Yang, Guohai; Li, He et al. (2015) Electrochemical sensors and biosensors based on nanomaterials and nanostructures. Anal Chem 87:230-49
Yang, Guohai; Zhu, Chengzhou; Du, Dan et al. (2015) Graphene-like two-dimensional layered nanomaterials: applications in biosensors and nanomedicine. Nanoscale 7:14217-31
Tanaka, Naoki; Takahashi, Shogo; Zhang, Yuan et al. (2015) Role of fibroblast growth factor 21 in the early stage of NASH induced by methionine- and choline-deficient diet. Biochim Biophys Acta 1852:1242-52
Tanaka, Naoki; Takahashi, Shogo; Fang, Zhong-Ze et al. (2014) Role of white adipose lipolysis in the development of NASH induced by methionine- and choline-deficient diet. Biochim Biophys Acta 1841:1596-607
Lee, Ai-Cheng; Du, Dan; Chen, Baowei et al. (2014) Electrochemical detection of leukemia oncogenes using enzyme-loaded carbon nanotube labels. Analyst 139:4223-30
Jin, Hongjun; Hallstrand, Teal S; Daly, Don S et al. (2014) A halotyrosine antibody that detects increased protein modifications in asthma patients. J Immunol Methods 403:17-25

Showing the most recent 10 out of 37 publications