Abstract

The overall goal of the Proteomics Initiative is to use mass spectrometry based proteomicmethods to expand the current migration knowledge database by examining phosphorylation site utilization ofproteins that regulate cell motility, by further developing methods for measuring differential phosphorylation ofthose proteins, and by using quantitative methods to assess positional and kinetic variation in phosphorylationsite utilization in migration-related proteins. In addition, the Initiative will extend these proteomic methods toprovide positional cartography of the proteins present in protein complexes formed by known migration-relatedproteins and protein products of novel migration genes identified in the unbiased screens of the GeneDiscovery Initiative. These goals are enabled by several pivotal developments in technology, analysis, andmethodology carried out during the first phase of funding. The Initiative focuses on two inter-related themes: 1.Phosphoproteomics of migration related proteins and 2. Positional proteomics of migration-related proteincomplexes and their phoshorylations.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
2U54GM064346-06
Application #
7313419
Study Section
Special Emphasis Panel (ZGM1-CBB-2 (GL))
Project Start
2006-08-10
Project End
2011-07-31
Budget Start
2006-08-10
Budget End
2007-07-31
Support Year
6
Fiscal Year
2006
Total Cost
$130,000
Indirect Cost

  Institution

Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Hu, Guiqing; Taylor, Dianne W; Liu, Jun et al. (2018) Identification of interfaces involved in weak interactions with application to F-actin-aldolase rafts. J Struct Biol 201:199-209
Kubow, Kristopher E; Shuklis, Victoria D; Sales, Dominic J et al. (2017) Contact guidance persists under myosin inhibition due to the local alignment of adhesions and individual protrusions. Sci Rep 7:14380
Gallegos, Lisa Leon; Ng, Mei Rosa; Sowa, Mathew E et al. (2016) A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ?-catenin. Sci Rep 6:27114
Al-Dimassi, Saleh; Salloum, Gilbert; Saykali, Bechara et al. (2016) Targeting the MAP kinase pathway in astrocytoma cells using a recombinant anthrax lethal toxin as a way to inhibit cell motility and invasion. Int J Oncol 48:1913-20
Juanes-Garcia, Alba; Chapman, Jessica R; Aguilar-Cuenca, Rocio et al. (2015) A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front-back polarity. J Cell Biol 209:23-32
Gao, Runchi; Zhao, Siwei; Jiang, Xupin et al. (2015) A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum. Sci Signal 8:ra50
Dai, Aguang; Ye, Feng; Taylor, Dianne W et al. (2015) The Structure of a Full-length Membrane-embedded Integrin Bound to a Physiological Ligand. J Biol Chem 290:27168-75
Márquez, G; Pinto, A; Alamo, L et al. (2014) A method for 3D-reconstruction of a muscle thick filament using the tilt series images of a single filament electron tomogram. J Struct Biol 186:265-72
Zgheib, Perla; Daher, Costantine F; Mroueh, Mohamad et al. (2014) Daucus carota Pentane/Diethyl Ether Fraction Inhibits Motility and Reduces Invasion of Cancer Cells. Chemotherapy 60:302-9
Hinde, Elizabeth; Yokomori, Kyoko; Gaus, Katharina et al. (2014) Fluctuation-based imaging of nuclear Rac1 activation by protein oligomerisation. Sci Rep 4:4219

Showing the most recent 10 out of 368 publications