The goal of the Imaging and Photomanipulation Initiative (I&PI) is to develop technologies formeasuring and perturbing the localized activities that mediate and regulate cell migration and to use thesetechnologies to make quantitative measurements for modeling cell migration. The emerging experimentalparadigm is to initially detect and quantify local molecular events in migrating cells and then to manipulate suchevents by local activation or inactivation and assess the consequences on the migratory process. The Initiativehas made great progress in developing the chemical and physical technologies and reagents to implement thisparadigm. The goals for the next granting period are not only to continue developing these technologies, butalso to use them on prototypical migration phenomena to develop working paradigms for studying cellularprocesses that are tightly regulated both spatially and temporally. The objectives of the I&PI are to: (1) developand test, in cell cultures, in micro-patterned cell cultures, and tissues, new biosensors and probes both for localphotoactivation and local photoinactivation, using chromophore assisted laser inactivation (CALI); (2) continuedevelopment of correlation methods for measuring molecular interactions, concentrations and dynamics both invitro and in tissues; and, (3) use the advances provided in objectives (1) and (2) to develop data useful formodeling migration phenomena by investigators both inside and outside the Consortium.
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