Abstract

The Cell Culture Core will provide state-of-the-art tissue and cell culture facilities to the individual projects of the Center for Reproductive Biological Research. This core will perform in vitro culture of immortalized and primary mammalian cells, murine embryonic stem cell manipulations and insect cell culture for baculoviral expression systems, as required by Center investigators and their staff. In addition, this facility will train core users in the use of in vitro cell culture techniques. The central facility will also purchase standard and specialized cell culture reagents which will improve reproducibility, time utilization and cost management. The core will be used 20% by Projects I and IV and 30% by Projects II and III.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
3U54HD007495-29S2
Application #
6564633
Study Section
Project Start
2001-04-01
Project End
2002-03-31
Budget Start
Budget End
Support Year
29
Fiscal Year
2002
Total Cost
$174,159
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Piyarathna, Danthasinghe Waduge Badrajee; Rajendiran, Thekkelnaycke M; Putluri, Vasanta et al. (2018) Distinct Lipidomic Landscapes Associated with Clinical Stages of Urothelial Cancer of the Bladder. Eur Urol Focus 4:907-915
Choi, Byung-Kwon; Dayaram, Tajhal; Parikh, Neha et al. (2018) Literature-based automated discovery of tumor suppressor p53 phosphorylation and inhibition by NEK2. Proc Natl Acad Sci U S A 115:10666-10671
Parikh, Neha; Shuck, Ryan L; Gagea, Mihai et al. (2018) Enhanced inflammation and attenuated tumor suppressor pathways are associated with oncogene-induced lung tumors in aged mice. Aging Cell 17:
Kotlajich, Matthew V; Xia, Jun; Zhai, Yin et al. (2018) Fluorescent fusions of the N protein of phage Mu label DNA damage in living cells. DNA Repair (Amst) 72:86-92
Szafran, Adam T; Stossi, Fabio; Mancini, Maureen G et al. (2017) Characterizing properties of non-estrogenic substituted bisphenol analogs using high throughput microscopy and image analysis. PLoS One 12:e0180141
Ha, Kyungsoo; Ma, Chengxian; Lin, Han et al. (2017) The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat Commun 8:15751
Roarty, K; Pfefferle, A D; Creighton, C J et al. (2017) Ror2-mediated alternative Wnt signaling regulates cell fate and adhesion during mammary tumor progression. Oncogene 36:5958-5968
Aagaard, Kjersti M; Lahon, Anismrita; Suter, Melissa A et al. (2017) Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication. Sci Rep 7:41389
Szafran, Adam T; Stephan, Cliff; Bolt, Michael et al. (2017) High-Content Screening Identifies Src Family Kinases as Potential Regulators of AR-V7 Expression and Androgen-Independent Cell Growth. Prostate 77:82-93
Tsai, Wei-Chih; Reineke, Lucas C; Jain, Antrix et al. (2017) Histone arginine demethylase JMJD6 is linked to stress granule assembly through demethylation of the stress granule-nucleating protein G3BP1. J Biol Chem 292:18886-18896

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