Abstract

GENE ARRAY FACILITY GOAL The long-term and continuing goal of the SCCPRR Gene Array Facility is to provide high quality, statistically robust gene expression data sets to members of the Specialized Cooperative Centers Program in Reproductive Research, the National Cooperative Program for Infertility Research, the Cooperative Reproductive Science Research Centers at Minority Institutions, and the Contraceptive Development Center. We believe the maintenance of an autonomous gene array facility specifically serving the NICHD reproductive research community is valuable for the following reasons: a) it allows overall costs of gene array analysis (from receipt of RNA to finished report) to be dramatically reduced, encouraging broad use of this new technology, and b) it allows us to provide critical support in the areas of experimental design, quality control, statistical analysis, and interpretation of biological significance.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54HD012629-30
Application #
7858460
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
2009-04-01
Budget End
2010-03-31
Support Year
30
Fiscal Year
2009
Total Cost
$213,251
Indirect Cost

  Institution

Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Chakraborty, Papia; Buaas, F William; Sharma, Manju et al. (2014) LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion. Stem Cells 32:860-73
Sanz, Elisenda; Evanoff, Ryan; Quintana, Albert et al. (2013) RiboTag analysis of actively translated mRNAs in Sertoli and Leydig cells in vivo. PLoS One 8:e66179
Cazorla, Maxime; Shegda, Mariya; Ramesh, Bhavani et al. (2012) Striatal D2 receptors regulate dendritic morphology of medium spiny neurons via Kir2 channels. J Neurosci 32:2398-409
Gill, John C; Navarro, Víctor M; Kwong, Cecilia et al. (2012) Increased neurokinin B (Tac2) expression in the mouse arcuate nucleus is an early marker of pubertal onset with differential sensitivity to sex steroid-negative feedback than Kiss1. Endocrinology 153:4883-93
Anawalt, Bradley D; Hotaling, James M; Walsh, Thomas J et al. (2012) Performance of total testosterone measurement to predict free testosterone for the biochemical evaluation of male hypogonadism. J Urol 187:1369-73
Navarro, Victor M; Ruiz-Pino, Francisco; Sanchez-Garrido, Miguel A et al. (2012) Role of neurokinin B in the control of female puberty and its modulation by metabolic status. J Neurosci 32:2388-97
Navarro, V M; Gottsch, M L; Wu, M et al. (2011) Regulation of NKB pathways and their roles in the control of Kiss1 neurons in the arcuate nucleus of the male mouse. Endocrinology 152:4265-75
Navarro, Victor M; Castellano, Juan M; McConkey, Sarah M et al. (2011) Interactions between kisspeptin and neurokinin B in the control of GnRH secretion in the female rat. Am J Physiol Endocrinol Metab 300:E202-10
Gottsch, Michelle L; Popa, Simina M; Lawhorn, Janessa K et al. (2011) Molecular properties of Kiss1 neurons in the arcuate nucleus of the mouse. Endocrinology 152:4298-309
Kim, Joshua; Semaan, Sheila J; Clifton, Donald K et al. (2011) Regulation of Kiss1 expression by sex steroids in the amygdala of the rat and mouse. Endocrinology 152:2020-30

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