The long term goals of this research project are to continue to address the problem of prefertilization immunocontraception from the perspective of a defined mammalian sperm surface component and its role in achieving contraception by the antigen's specific antibody interfering with fertilization. It is our goal to define at the molecular and physiological level a set of sperm molecules which functions during fertilization and whose primary sequences can be engineered into an effective immunocontraceptive. Hence, a blending of molecular biology and sperm physiology will lead us to a better understanding of precise targets for contraception. To continue this line of inquiry, the current proposals's specific aims are directed toward studies on the effect on fertility of mice and monkeys immunized with constructs derived from human Sp17 (rHSp17), and a combination of rHSp17 and zona pellucida (ZP) constructs.
Aim number 1: Fertility testing in mice and immunogenicity testing in monkeys of chimeric peptides consisting of a promiscuous T-cell epitope and a rHSp17 peptide synthesized as a single synthetic peptide.
Aim number 2: Fertility testing in mice and immunogenicity testing in monkeys of recombinant proteins which represent portions of the entire HSp17 sequence that contain specific immunodominant epitopes and portions of the zona pellucida sequence which represent the sperm binding site(s).
Aim number 3: A fertility trial of 15 immunized monkeys and 15 control monkeys.
Aim number 4: the physiological characterization of human Sp17 by examining in detail its expression in a eukaryotic cell.
Aim number 5: An examination of the effect on fertility in mice immunized with two different recombinant proteins from sperm and zona pellucida.

Project Start
1999-03-01
Project End
2000-02-29
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
8
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Virginia
Department
Type
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Pires, Eusebio S; Hlavin, Callie; Macnamara, Ellen et al. (2013) SAS1B protein [ovastacin] shows temporal and spatial restriction to oocytes in several eutherian orders and initiates translation at the primary to secondary follicle transition. Dev Dyn 242:1405-26
Shumilin, Igor A; Cymborowski, Marcin; Chertihin, Olga et al. (2012) Identification of unknown protein function using metabolite cocktail screening. Structure 20:1715-25
Naaby-Hansen, Soren; Herr, John C (2010) Heat shock proteins on the human sperm surface. J Reprod Immunol 84:32-40
Naaby-Hansen, Soren; Diekman, Alan; Shetty, Jagathpala et al. (2010) Identification of calcium-binding proteins associated with the human sperm plasma membrane. Reprod Biol Endocrinol 8:6
Wolkowicz, M J; Digilio, L; Klotz, K et al. (2008) Equatorial segment protein (ESP) is a human alloantigen involved in sperm-egg binding and fusion. J Androl 29:272-82
Klotz, Kenneth L; Coppola, Michael A; Labrecque, Michel et al. (2008) Clinical and consumer trial performance of a sensitive immunodiagnostic home test that qualitatively detects low concentrations of sperm following vasectomy. J Urol 180:2569-76
Kurth, Barbara E; Digilio, Laura; Snow, Phillip et al. (2008) Immunogenicity of a multi-component recombinant human acrosomal protein vaccine in female Macaca fascicularis. J Reprod Immunol 77:126-41
Herr, John C; Chertihin, Olga; Digilio, Laura et al. (2008) Distribution of RNA binding protein MOEP19 in the oocyte cortex and early embryo indicates pre-patterning related to blastomere polarity and trophectoderm specification. Dev Biol 314:300-16
Shetty, Jagathpala; Bronson, Richard A; Herr, John C (2008) Human sperm protein encyclopedia and alloantigen index: mining novel allo-antigens using sera from ASA-positive infertile patients and vasectomized men. J Reprod Immunol 77:23-31
Xu, Bingfang; Hao, Zhonglin; Jha, Kula N et al. (2008) TSKS concentrates in spermatid centrioles during flagellogenesis. Dev Biol 319:201-10

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