Abstract

A. Objective The objective of Core C is to develop and disseminate experimental and computational technologies that are critical for studying the molecular genetics and genomics of small biological samples, as exemplified by germ cell or pre-implantation embryo samples. We will take two main directions to accomplish this objective:
Aim 1. Develop experimental protocols to apply massively parallel RNA-sequencing (RNA-Seq) to discover novel, splice and other 3'UTR variants and their differential roles in development, with a special emphasis on understanding the post-transcriptional regulatory function of 3'UTR variants.
Aim 2. Host and disseminate validated experimental protocols, computational tools and annotated RNA-Seq data sets to make next-generation sequencing, single-cell analysis, and microfluidics techniques accessible to the Reproductive Biology, SCCPRIR, and broader scientific communities.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54HD068158-03
Application #
8446124
Study Section
Special Emphasis Panel (ZHD1-DSR-L)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
3
Fiscal Year
2013
Total Cost
$185,959
Indirect Cost
$74,147

  Institution

Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Gustin, Stephanie L F; Wang, Guangwen; Baker, Valerie M et al. (2018) Use of human-derived stem cells to create a novel, in vitro model designed to explore FMR1 CGG repeat instability amongst female premutation carriers. J Assist Reprod Genet :
Woglar, Alexander; Villeneuve, Anne M (2018) Dynamic Architecture of DNA Repair Complexes and the Synaptonemal Complex at Sites of Meiotic Recombination. Cell 173:1678-1691.e16
Feng, Yi; Cui, Peng; Lu, Xiaowei et al. (2017) CLARITY reveals dynamics of ovarian follicular architecture and vasculature in three-dimensions. Sci Rep 7:44810
Karakikes, Ioannis; Termglinchan, Vittavat; Cepeda, Diana A et al. (2017) A Comprehensive TALEN-Based Knockout Library for Generating Human-Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases. Circ Res 120:1561-1571
Feng, Yi; Zhu, Shoujun; Antaris, Alexander L et al. (2017) Live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared II fluorophore. Chem Sci 8:3703-3711
Fan, Qianlan; Cheng, Yuan; Chang, Hsun-Ming et al. (2017) Sphingosine-1-phosphate promotes ovarian cancer cell proliferation by disrupting Hippo signaling. Oncotarget 8:27166-27176
Panula, Sarita; Reda, Ahmed; Stukenborg, Jan-Bernd et al. (2016) Over Expression of NANOS3 and DAZL in Human Embryonic Stem Cells. PLoS One 11:e0165268
Kawamura, Kazuhiro; Kawamura, Nanami; Hsueh, Aaron J W (2016) Activation of dormant follicles: a new treatment for premature ovarian failure? Curr Opin Obstet Gynecol 28:217-22
Durruthy-Durruthy, Jens; Sebastiano, Vittorio; Wossidlo, Mark et al. (2016) The primate-specific noncoding RNA HPAT5 regulates pluripotency during human preimplantation development and nuclear reprogramming. Nat Genet 48:44-52
Zhai, Jun; Yao, Guidong; Dong, Fangli et al. (2016) In Vitro Activation of Follicles and Fresh Tissue Auto-transplantation in Primary Ovarian Insufficiency Patients. J Clin Endocrinol Metab 101:4405-4412

Showing the most recent 10 out of 39 publications