The major aim of the Imaging and Microscopy Core is to facilitate the research of investigators by providingaccess to imaging and microscopy equipment that might be expensive to procure and maintain in anindividual investigator's laboratory not dedicated to these techniques. This allows investigators to broadenthe techniques available for their research within the Neuroscience Institute by allowing the burden ofexpenses to be shared between individual investigators, the Neuroscience Institute and Morehouse Schoolof Medicine.The imaging core can provide equipment for brightfield, fluorescence and confocal microscopy and imageacquisition. The hardware and software are designed to allow production and analysis of images associatedwith in situ hybridization, immunocytochemistry, histology, as well as digitization and analysis of gelsproduced from molecular techniques such as Northerns, Westerns, Southerns, PCR and RT-PCR. Imagesproduced are publication quality.Among the U54 participants, the Davidson laboratory will use the cryostat and microscope imagingequipment of the core to perform pathological analyses on tissue samples harvested from mice on shiftinglight schedules. The Paul laboratory will use the cryostat in the imaging core to section brain tissue for in situhybridization and subsequent optical density analysis of autoradiograph images. In addition, the Paullaboratory will use confocal microscopy to assist in mapping expression of the GABAA subunit expression inthe suprachiasmatic nucleus and other hypothalamic nuclei using novel GABAA subunit antibodies. TheFukuhara laboratory will use brightfield and fluorescence microscopy to examine whether Bmall-luciferaseDNA is successfully delivered to fibroblasts utilizing in situ hybridization and image analysis softwareprovided by the core. Core equipment can also be used, under proper guidance by other NeuroscienceInstitute investigators and other Morehouse School of Medicine personnel.
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