Abstract

The goal of this project is to understand mechanisms of host defense and immunity against retroviruses. Previous work has defined several mouse genes which influence ability of mice to recover spontaneously from Friend virus complex (FV)-induced leukemia. Some of these genes also influence ability to induce protective immunity to this disease by vaccination with viral protein in adjuvants or expressed in recombinant vaccinia viruses. The most recent work has shown that protective immunity can be induced by both retroviral envelope and gag proteins expressed in vaccinia. Deletion of the amino or carboxy-termini of the gag polyprotein indicated that the amino-terminal half was responsible for the immune protection. This protection was weaker than that induced by the viral envelope protein, but it was successful in mice who were not capable of making a successful immune response to the envelope protein. These results suggested that both envelope and gag proteins should be considered as possible immunogens in constructing vaccines against other retroviruses. The mechanism of protection by immunization with gag protein is under further investigation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000074-18
Application #
3809543
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
18
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Mehaffey, J Hunter; Michaels, Alex D; Mullen, Mathew G et al. (2017) Patient travel for bariatric surgery: does distance matter? Surg Obes Relat Dis 13:2027-2031
Michaels, Alex D; Mullen, Matthew G; Guidry, Christopher A et al. (2017) Unplanned Reoperation Following Colorectal Surgery: Indications and Operations. J Gastrointest Surg 21:1480-1485
Michaels, Alex D; Hunter Mehaffey, J; Brenton French, W et al. (2017) Hypoglycemia Following Bariatric Surgery: Our 31-Year Experience. Obes Surg 27:3118-3123
Mehaffey, J Hunter; Hawkins, Robert B; Mullen, Matthew G et al. (2017) Access to Quaternary Care Surgery: Implications for Accountable Care Organizations. J Am Coll Surg 224:525-529
Mullen, Matthew G; Salerno, Elise P; Michaels, Alex D et al. (2016) Declining Operative Experience for Junior-Level Residents: Is This an Unintended Consequence of Minimally Invasive Surgery? J Surg Educ 73:609-15
Brien, James D; Daffis, Stephane; Lazear, Helen M et al. (2011) Interferon regulatory factor-1 (IRF-1) shapes both innate and CD8(+) T cell immune responses against West Nile virus infection. PLoS Pathog 7:e1002230
Peterson, Karin E; Pourciau, Susan; Du, Min et al. (2008) Neurovirulence of polytropic murine retrovirus is influenced by two separate regions on opposite sides of the envelope protein receptor binding domain. J Virol 82:8906-10
Santiago, Mario L; Montano, Mauricio; Benitez, Robert et al. (2008) Apobec3 encodes Rfv3, a gene influencing neutralizing antibody control of retrovirus infection. Science 321:1343-6
Peterson, K E; Chesebro, B (2006) Influence of proinflammatory cytokines and chemokines on the neuropathogenesis of oncornavirus and immunosuppressive lentivirus infections. Curr Top Microbiol Immunol 303:67-95
Peterson, Karin E; Evans, Leonard H; Wehrly, Kathy et al. (2006) Increased proinflammatory cytokine and chemokine responses and microglial infection following inoculation with neural stem cells infected with polytropic murine retroviruses. Virology 354:143-53

Showing the most recent 10 out of 24 publications