Abstract

For the past several years wwe having been studying brain disease induced by the Fr98 strain of mouse retrovirus. In FY08 our experiments identified the amino acid residues in the envelope of the Fr98 mouse retrovirus which were required for the brain disease induced by this virus. These residues were located in several separate locations in the linear protein sequence. When the envelope preotein structure was viewed in 3-dimensions based on the crystal structure of a closely related mouse retrovirus, these amino acids were located in two distinct groups. These results supported the idea that two separate areas of the surface of the envelope protein cooperatively influence the neuropathogenesis of this retrovirus.? ? In FY08 our group collaborated with the laboratories of Dr Kim Hasenkrug at LPVD and Dr Warner Greene at UCSF to demonstrate that the Rfv-3 gene discovered in 1978 at LPVD was identical to the APOBEC3 gene which is currently under intense study in the HIV field. These results indicated that APOBEC3 appeared to have previously unsuspected effects on the immune response to retroviruses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000074-36
Application #
7732418
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
36
Fiscal Year
2008
Total Cost
$186,853
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Mehaffey, J Hunter; Michaels, Alex D; Mullen, Mathew G et al. (2017) Patient travel for bariatric surgery: does distance matter? Surg Obes Relat Dis 13:2027-2031
Michaels, Alex D; Mullen, Matthew G; Guidry, Christopher A et al. (2017) Unplanned Reoperation Following Colorectal Surgery: Indications and Operations. J Gastrointest Surg 21:1480-1485
Michaels, Alex D; Hunter Mehaffey, J; Brenton French, W et al. (2017) Hypoglycemia Following Bariatric Surgery: Our 31-Year Experience. Obes Surg 27:3118-3123
Mehaffey, J Hunter; Hawkins, Robert B; Mullen, Matthew G et al. (2017) Access to Quaternary Care Surgery: Implications for Accountable Care Organizations. J Am Coll Surg 224:525-529
Mullen, Matthew G; Salerno, Elise P; Michaels, Alex D et al. (2016) Declining Operative Experience for Junior-Level Residents: Is This an Unintended Consequence of Minimally Invasive Surgery? J Surg Educ 73:609-15
Brien, James D; Daffis, Stephane; Lazear, Helen M et al. (2011) Interferon regulatory factor-1 (IRF-1) shapes both innate and CD8(+) T cell immune responses against West Nile virus infection. PLoS Pathog 7:e1002230
Peterson, Karin E; Pourciau, Susan; Du, Min et al. (2008) Neurovirulence of polytropic murine retrovirus is influenced by two separate regions on opposite sides of the envelope protein receptor binding domain. J Virol 82:8906-10
Santiago, Mario L; Montano, Mauricio; Benitez, Robert et al. (2008) Apobec3 encodes Rfv3, a gene influencing neutralizing antibody control of retrovirus infection. Science 321:1343-6
Peterson, K E; Chesebro, B (2006) Influence of proinflammatory cytokines and chemokines on the neuropathogenesis of oncornavirus and immunosuppressive lentivirus infections. Curr Top Microbiol Immunol 303:67-95
Peterson, Karin E; Evans, Leonard H; Wehrly, Kathy et al. (2006) Increased proinflammatory cytokine and chemokine responses and microglial infection following inoculation with neural stem cells infected with polytropic murine retroviruses. Virology 354:143-53

Showing the most recent 10 out of 24 publications