Immunochemistry of Parasitic Diseases

Nash, T E.

Abstract

The number of variant specific proteins (VSPs) in one Giardia clone was estimated to be between 130 and 150. Four copies of each gene appear to present and therefore between 30-40 different VSPs exist in this clone. Electron microscopy studies showed some Giardia lack a surface coat where VSPs are localized. Giardia infected athymic mice undergo antigenic variation but SCID mice do not suggesting non-cellular processes are responsible for loss of the initial VSP. HIV can enter and survive in Giardia; however, it is not clear if viable HIV are produced.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000161-14
• Application #: 3803100
• Support Year: 14
• Fiscal Year: 1991

Institution

• Name: National Institute of Allergy and Infectious Diseases
• Country: United States

Publications

Kulakova, Liudmila; Singer, Steven M; Conrad, John et al. (2006) Epigenetic mechanisms are involved in the control of Giardia lamblia antigenic variation. Mol Microbiol 61:1533-42

Hayman, J Russell; Southern, Timothy R; Nash, Theodore E (2005) Role of sulfated glycans in adherence of the microsporidian Encephalitozoon intestinalis to host cells in vitro. Infect Immun 73:841-8

Elmendorf, Heidi G; Rohrer, Sally C; Khoury, Rasha S et al. (2005) Examination of a novel head-stalk protein family in Giardia lamblia characterised by the pairing of ankyrin repeats and coiled-coil domains. Int J Parasitol 35:1001-11

Touz, Maria C; Kulakova, Liudmila; Nash, Theodore E (2004) Adaptor protein complex 1 mediates the transport of lysosomal proteins from a Golgi-like organelle to peripheral vacuoles in the primitive eukaryote Giardia lamblia. Mol Biol Cell 15:3053-60

Worgall, Tilla S; Davis-Hayman, Sara R; Magana, Marissa M et al. (2004) Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter: evidence for SREBP as an ancient transcription factor. J Lipid Res 45:981-8

Zhou, Ping; Li, Erqiu; Zhu, Nannan et al. (2003) Role of interleukin-6 in the control of acute and chronic Giardia lamblia infections in mice. Infect Immun 71:1566-8

Hinshaw, Jerald C; Suh, Dae-Yeon; Garnier, Philippe et al. (2003) Oxidosqualene cyclase inhibitors as antimicrobial agents. J Med Chem 46:4240-3

Touz, Maria C; Lujan, Hugo D; Hayes, Stanley F et al. (2003) Sorting of encystation-specific cysteine protease to lysosome-like peripheral vacuoles in Giardia lamblia requires a conserved tyrosine-based motif. J Biol Chem 278:6420-6

Davis-Hayman, Sara R; Hayman, J Russell; Nash, Theodore E (2003) Encystation-specific regulation of the cyst wall protein 2 gene in Giardia lamblia by multiple cis-acting elements. Int J Parasitol 33:1005-12

Touz, Maria C; Nores, Maria J; Slavin, Ileana et al. (2002) The activity of a developmentally regulated cysteine proteinase is required for cyst wall formation in the primitive eukaryote Giardia lamblia. J Biol Chem 277:8474-81