Abstract

Purpose: 1. To identify the receptors on malaria parasites for red cell invasion and on infected red cells for adhesion to endothelium and to placenta. 2. To identify the red cell receptors for each parasite ligand. 3. To identify the minimal domain in the variant antigen for binding to placenta. 4. Test a region on the variant antigen for inducing protection against P. falciparum in Aotus monkeys and for overcoming variation. Accomplishments during the year: 1. The BAEBL receptor is blocked by purified glycophorin C. 2. The receptor domain of AMA1 for binding red cells has been identified as well as the protein on the red cell for binding of AMA1. 3. The difference between Dd2 (no invasion of neuraminidase treated red cells) and Dd2/nm (invasion of neuraminidase treated red cells) has been identified. 4. The domain on infected red cells for binding CSA has been identified on multiple DBL domains.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000241-23
Application #
6984937
Study Section
(LMVR)
Project Start
Project End
Budget Start
Budget End
Support Year
23
Fiscal Year
2004
Total Cost
Indirect Cost

  Institution

Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Mayer, D C Ghislaine; Cofie, Joann; Jiang, Lubin et al. (2009) Glycophorin B is the erythrocyte receptor of Plasmodium falciparum erythrocyte-binding ligand, EBL-1. Proc Natl Acad Sci U S A 106:5348-52
Beaty, Barry J; Prager, Denis J; James, Anthony A et al. (2009) From tucson to genomics and transgenics: the vector biology network and the emergence of modern vector biology. PLoS Negl Trop Dis 3:e343
Qian, Feng; Rausch, Kelly M; Muratova, Olga et al. (2008) Addition of CpG ODN to recombinant Pseudomonas aeruginosa ExoProtein A conjugates of AMA1 and Pfs25 greatly increases the number of responders. Vaccine 26:2521-7
Miura, Kazutoyo; Orcutt, Andrew C; Muratova, Olga V et al. (2008) Development and characterization of a standardized ELISA including a reference serum on each plate to detect antibodies induced by experimental malaria vaccines. Vaccine 26:193-200
Dicko, Alassane; Sagara, Issaka; Ellis, Ruth D et al. (2008) Phase 1 study of a combination AMA1 blood stage malaria vaccine in Malian children. PLoS ONE 3:e1563
Huaman, Maria Cecilia; Martin, Laura B; Malkin, Elissa et al. (2008) Ex vivo cytokine and memory T cell responses to the 42-kDa fragment of Plasmodium falciparum merozoite surface protein-1 in vaccinated volunteers. J Immunol 180:1451-61
Wu, Yimin; Ellis, Ruth D; Shaffer, Donna et al. (2008) Phase 1 trial of malaria transmission blocking vaccine candidates Pfs25 and Pvs25 formulated with montanide ISA 51. PLoS ONE 3:e2636
Mullen, Gregory E D; Ellis, Ruth D; Miura, Kazutoyo et al. (2008) Phase 1 trial of AMA1-C1/Alhydrogel plus CPG 7909: an asexual blood-stage vaccine for Plasmodium falciparum malaria. PLoS ONE 3:e2940
Dicko, Alassane; Sagara, Issaka; Diemert, David et al. (2007) Year-to-year variation in the age-specific incidence of clinical malaria in two potential vaccine testing sites in Mali with different levels of malaria transmission intensity. Am J Trop Med Hyg 77:1028-33
Qian, Feng; Wu, Yimin; Muratova, Olga et al. (2007) Conjugating recombinant proteins to Pseudomonas aeruginosa ExoProtein A: a strategy for enhancing immunogenicity of malaria vaccine candidates. Vaccine 25:3923-33

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