In 1980 we described a new form of viral hepatitis caused by a previously unrecognized hepatitis virus with epidemiologic characteristics of HAV (i.e., the virus is fecal-orally transmitted, produces water-borne epidemics and is probably responsible for a significant proportion of endemic hepatitis) but not related to HAV. The disease has been called epidemic or enterically transmitted non-A, non-B hepatitis (ENANB). Such hepatitis is both epidemic and endemic throughout the Indian subcontinent, central Asia, parts of the Middle East and northern Africa, with probable extension to West Africa. The putative etiologic agent, a 27-30nm nonenveloped virus, was identified in a volunteer study in 1983 and similar virus-like particles have been detected in feces of cases in a number of other outbreaks. The small quantity of such virus-like particles available for study has limited progress The objectives of this study are to identify and characterize the etiologic agent of ENANB hepatitis and to develop useful assays for diagnosis of infection and seroepidemiologic studies. A longer term objective is the development of passive and active immunoprophylaxis for this important human pathogen. The identification of an acute phase stool sample that contains marginally sufficient virus-like particles for use as an antigen in immune electronmicroscopic studies of ENANB has permitted limited cross-serologic studies of paired sera obtained from epidemic and endemic disease in regions where the virus is prevalent. From these studies we conclude that ENANB hepatitis occurring throughout India, in Pakistan, southern Russia and Algeria is all caused by serologically related or identical viruses. Chimpanzees inoculated with stools containing virus-like particles from the outbreaks developed hepatitis and seroconverted, thus proving transmissibility to a non-human primate species.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000311-07
Application #
3818184
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Christensen, Peer B; Engle, Ronald E; Hjort, Charlotte et al. (2008) Time trend of the prevalence of hepatitis E antibodies among farmers and blood donors: a potential zoonosis in Denmark. Clin Infect Dis 47:1026-31
Purcell, R H; Emerson, S U (2008) Hepatitis E: an emerging awareness of an old disease. J Hepatol 48:494-503
Easterbrook, J D; Kaplan, J B; Vanasco, N B et al. (2007) A survey of zoonotic pathogens carried by Norway rats in Baltimore, Maryland, USA. Epidemiol Infect 135:1192-9
Yu, Claro; Zimmerman, Carl; Stone, Roger et al. (2007) Using improved technology for filter paper-based blood collection to survey wild Sika deer for antibodies to hepatitis E virus. J Virol Methods 142:143-50
Meky, Fatma A; Stoszek, Sonia K; Abdel-Hamid, Mohamed et al. (2006) Active surveillance for acute viral hepatitis in rural villages in the Nile Delta. Clin Infect Dis 42:628-33
Stoszek, Sonia K; Abdel-Hamid, Mohamed; Saleh, Doa'a A et al. (2006) High prevalence of hepatitis E antibodies in pregnant Egyptian women. Trans R Soc Trop Med Hyg 100:95-101
Stoszek, Sonia K; Engle, Ronald E; Abdel-Hamid, Mohamed et al. (2006) Hepatitis E antibody seroconversion without disease in highly endemic rural Egyptian communities. Trans R Soc Trop Med Hyg 100:89-94
Graff, Judith; Nguyen, Hanh; Yu, Claro et al. (2005) The open reading frame 3 gene of hepatitis E virus contains a cis-reactive element and encodes a protein required for infection of macaques. J Virol 79:6680-9
Graff, Judith; Nguyen, Hanh; Kasorndorkbua, Chaiyan et al. (2005) In vitro and in vivo mutational analysis of the 3'-terminal regions of hepatitis e virus genomes and replicons. J Virol 79:1017-26
Emerson, Suzanne U; Arankalle, Vidya A; Purcell, Robert H (2005) Thermal stability of hepatitis E virus. J Infect Dis 192:930-3

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