Two live attenuated PIV3 candidate vaccines are in Phase 1-2 trials in human infants and children, namely the cold-passaged 45 mutant of the JS wildtype human PIV3 and the antigenically related bovine PIV3. A complete cDNA copy of the JS strain of human PIV3 has been constructed. This cDNA has the exact coding sequence of the wild type virus except for engineered mutations that will permit the transfectant virus to escape neutralization by monoclonal antibodies that neutralize the infectivity of the helper virus. With this cDNA, it is now possible in the coming year to attempt to rescue infectious virus from RNA generated from this cDNA.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000327-13
Application #
3746510
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
1994
Total Cost
Indirect Cost
City
State
Country
United States
Zip Code
Boonyaratanakornkit, Jim B; Bartlett, Emmalene J; Amaro-Carambot, Emerito et al. (2009) The C proteins of human parainfluenza virus type 1 (HPIV1) control the transcription of a broad array of cellular genes that would otherwise respond to HPIV1 infection. J Virol 83:1892-910
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