Isolation and Serotypic Characterization of Human and Animal Rotaviruses

Hoshino, Y.

Abstract

In this project we determined the major antigenic relationships of various rotavirus strains derived from humans and animals. Elucidation of the neutralization specificities of rotavirus is important in order to achieve a more comprehensive understanding of rotavirus epidemiology and for formulation of an effective strategy for vaccination. For example: (i) the bovine rotavirus strain NCDV-Cody which was originally isolated from a diarrheic calf and traditionally considered as the virulent counterpart for the attenuated NCDV-Lincoln (VP7 serotype 6) was shown to carry VP7 serotype 8 and VP4 serotype 6; (ii) the human rotavirus strain 69M (VP7 serotype 8) which was originally isolated from a child with diarrhea in Indonesia, was found to possess a new VP4 neutralization specificity which was closely related antigenically to equine rotavirus H-2 VP4; (iii) the human rotavirus strain MT19 which was originally isolated from a diarrheic child in Brazil was found to have a strong two-way cross-neutralization relationship with porcine rotavirus serotype 5 (OSU strain) and serotype 11 (YM strain); (iv) the vervet monkey rotavirus strain SA11 was shown to possess a distinct VP4 neutralization specificity which was related in a one-way fashion to rhesus monkey rotavirus MMU18006 VP4.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000339-14
• Application #: 5200442
• Support Year: 14
• Fiscal Year: 1995

Institution

• Name: National Institute of Allergy and Infectious Diseases
• Country: United States

Publications

Ross, Jerri; Ostlund, Eileen N; Cao, Dianjun et al. (2008) Acrylamide concentration affects the relative position of VP7 gene of serotype G2 strains as determined by polyacrylamide gel electrophoresis. J Clin Virol 42:374-80

Cao, Dianjun; Santos, Norma; Jones, Ronald W et al. (2008) The VP7 genes of two G9 rotaviruses isolated in 1980 from diarrheal stool samples collected in Washington, DC, are unique molecularly and serotypically. J Virol 82:4175-9

Santos, Norma; Honma, Shinjiro; Timenetsky, Maria do Carmo S T et al. (2008) Development of a microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay for identification of clinically relevant human group A rotavirus G and P genotypes. J Clin Microbiol 46:462-9

Honma, Shinjiro; Chizhikov, Vladimir; Santos, Norma et al. (2007) Development and validation of DNA microarray for genotyping group A rotavirus VP4 (P[4], P[6], P[8], P[9], and P[14]) and VP7 (G1 to G6, G8 to G10, and G12) genes. J Clin Microbiol 45:2641-8

Volotao, Eduardo M; Soares, Caroline C; Maranhao, Adriana G et al. (2006) Rotavirus surveillance in the city of Rio de Janeiro-Brazil during 2000-2004: detection of unusual strains with G8P[4] or G10P[9] specificities. J Med Virol 78:263-72

Hoshino, Yasutaka; Honma, Shinjiro; Jones, Ronald W et al. (2006) A rotavirus strain isolated from pig-tailed macaque (Macaca nemestrina) with diarrhea bears a P6[1]:G8 specificity. Virology 345:1-12

Pietruchinski, Eduardo; Benati, Fabricio; Lauretti, Flavio et al. (2006) Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: distribution of G/P types and finding of a rare G12 strain. J Med Virol 78:1241-9

Hoshino, Yasutaka; Jones, Ronald W; Ross, Jerri et al. (2005) Porcine rotavirus strain Gottfried-based human rotavirus candidate vaccines: construction and characterization. Vaccine 23:3791-9

Santos, Norma; Volotao, Eduardo M; Soares, Caroline C et al. (2005) Predominance of rotavirus genotype G9 during the 1999, 2000, and 2002 seasons among hospitalized children in the city of Salvador, Bahia, Brazil: implications for future vaccine strategies. J Clin Microbiol 43:4064-9

Kapikian, Albert Z; Simonsen, Lone; Vesikari, Timo et al. (2005) A hexavalent human rotavirus-bovine rotavirus (UK) reassortant vaccine designed for use in developing countries and delivered in a schedule with the potential to eliminate the risk of intussusception. J Infect Dis 192 Suppl 1:S22-9