In this project we determined the antigenic relationships based on VP4 and VP7 neutralization specificities of various rotavirus strains derived from humans and animals. The elucidation of the neutralization specificities of rotaviruses is important in order to achieve a more comprehensive understanding of rotavirus epidemiology and for formulation of an effective strategy for vaccination. By using various single gene substitution human x human or human x animal rotavirus reassortants and hyperimmune guinea pig antiserum raised against each reassortant, we determined that (i) human rotavirus strain Se585 which was isolated in Seattle, WA, during strain surveillance conducted by CDC, belonged to an uncommon G12 serotype that had been isolated thus far only in the Philippines, (ii) strains CC425 and KC814 carried P3[9] specificity, which was the first detection in the US of a P[9] virus, and (iii) a small outbreak of diarrhea (n=16) in Rio de Janeiro, Brazil, in 1998-1999 was caused by a rare G3,P3[9] virus (Santos). In addition, we characterized VP4 neutralization specificities of a porcine rotavirus Gottfried strain (P1B[6],G4) and showed there was a one-way relationship between Gottfreid VP4 and human rotavirus VP4 with P[6] specificity, in which the human rotavirus was the prime strain. In addition, in collaboration with Food and Drug Administration (Chizhikov), we have established an oligonucleotide microarray assay to G genotype human rotaviruses.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000339-21
Application #
6669360
Study Section
(LID)
Project Start
Project End
Budget Start
Budget End
Support Year
21
Fiscal Year
2002
Total Cost
Indirect Cost
Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Ross, Jerri; Ostlund, Eileen N; Cao, Dianjun et al. (2008) Acrylamide concentration affects the relative position of VP7 gene of serotype G2 strains as determined by polyacrylamide gel electrophoresis. J Clin Virol 42:374-80
Cao, Dianjun; Santos, Norma; Jones, Ronald W et al. (2008) The VP7 genes of two G9 rotaviruses isolated in 1980 from diarrheal stool samples collected in Washington, DC, are unique molecularly and serotypically. J Virol 82:4175-9
Santos, Norma; Honma, Shinjiro; Timenetsky, Maria do Carmo S T et al. (2008) Development of a microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay for identification of clinically relevant human group A rotavirus G and P genotypes. J Clin Microbiol 46:462-9
Honma, Shinjiro; Chizhikov, Vladimir; Santos, Norma et al. (2007) Development and validation of DNA microarray for genotyping group A rotavirus VP4 (P[4], P[6], P[8], P[9], and P[14]) and VP7 (G1 to G6, G8 to G10, and G12) genes. J Clin Microbiol 45:2641-8
Volotao, Eduardo M; Soares, Caroline C; Maranhao, Adriana G et al. (2006) Rotavirus surveillance in the city of Rio de Janeiro-Brazil during 2000-2004: detection of unusual strains with G8P[4] or G10P[9] specificities. J Med Virol 78:263-72
Hoshino, Yasutaka; Honma, Shinjiro; Jones, Ronald W et al. (2006) A rotavirus strain isolated from pig-tailed macaque (Macaca nemestrina) with diarrhea bears a P6[1]:G8 specificity. Virology 345:1-12
Pietruchinski, Eduardo; Benati, Fabricio; Lauretti, Flavio et al. (2006) Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: distribution of G/P types and finding of a rare G12 strain. J Med Virol 78:1241-9
Hoshino, Yasutaka; Jones, Ronald W; Ross, Jerri et al. (2005) Porcine rotavirus strain Gottfried-based human rotavirus candidate vaccines: construction and characterization. Vaccine 23:3791-9
Santos, Norma; Volotao, Eduardo M; Soares, Caroline C et al. (2005) Predominance of rotavirus genotype G9 during the 1999, 2000, and 2002 seasons among hospitalized children in the city of Salvador, Bahia, Brazil: implications for future vaccine strategies. J Clin Microbiol 43:4064-9
Kapikian, Albert Z; Simonsen, Lone; Vesikari, Timo et al. (2005) A hexavalent human rotavirus-bovine rotavirus (UK) reassortant vaccine designed for use in developing countries and delivered in a schedule with the potential to eliminate the risk of intussusception. J Infect Dis 192 Suppl 1:S22-9

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