Experimental Studies in Animals with Various Rotaviruses and Their Reassortants

Hoshino, Y.

Abstract

The aims of this project are to evaluate virulence and immune response of selected human and animal rotaviruses in experimentally infected animals, and to study cross-protection between selected strains of human and animal rotaviruses. Study of potential vaccine strains in experimental animals also constitutes an additional test for the presence of adventitious agents. Experimental animal systems that are currently available and that have been shown to be suitable for the study of rotavirus infection and in some cases disease, are the colostrum-deprived newborn rhesus monkey and the gnotobiotic piglet. Chimpanzees, rhesus monkeys and African green monkeys are also utilized to examine the safety of potential rotavirus vaccine preparations. Piglets were delivered by Caesarean section and maintained in plastic isolators under gnotobiotic conditions; monkeys were maintained under strict isolation in contained units. Sera and fecal samples derived from the animal studies were analyzed for antibody and antigen by a variety of laboratory techniques. Enzyme immunoassay was used to evaluate fecal samples for antigen, while complement fixation, immune adherence hemagglutination assay and/or plaque reduction neutralization assay were used to evaluate sera and fecal samples for antibody response.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000340-04
• Application #: 4688479
• Support Year: 4
• Fiscal Year: 1985

Institution

• Name: Niaid Extramural Activities
• Country: United States

Publications

Feng, N; Sen, A; Nguyen, H et al. (2009) Variation in antagonism of the interferon response to rotavirus NSP1 results in differential infectivity in mouse embryonic fibroblasts. J Virol 83:6987-94

Ross, Jerri; Ostlund, Eileen N; Cao, Dianjun et al. (2008) Acrylamide concentration affects the relative position of VP7 gene of serotype G2 strains as determined by polyacrylamide gel electrophoresis. J Clin Virol 42:374-80

Kapikian, Albert Z; Hoshino, Yasutaka (2007) To serotype or not to serotype: that is still the question. J Infect Dis 195:611-4

Vesikari, Timo; Karvonen, Aino V; Majuri, Jukka et al. (2006) Safety, Efficacy, and Immunogenicity of 2 Doses of Bovine-Human (UK) and Rhesus-Rhesus-Human Rotavirus Reassortant Tetravalent Vaccines in Finnish Children. J Infect Dis 194:370-6

Vesikari, Timo; Karvonen, Aino; Forrest, Bruce D et al. (2006) Neonatal administration of rhesus rotavirus tetravalent vaccine. Pediatr Infect Dis J 25:118-22

Hoshino, Yasutaka; Honma, Shinjiro; Jones, Ronald W et al. (2005) A porcine G9 rotavirus strain shares neutralization and VP7 phylogenetic sequence lineage 3 characteristics with contemporary human G9 rotavirus strains. Virology 332:177-88

Hoshino, Yasutaka; Jones, Ronald W; Ross, Jerri et al. (2005) Porcine rotavirus strain Gottfried-based human rotavirus candidate vaccines: construction and characterization. Vaccine 23:3791-9

Kapikian, Albert Z; Simonsen, Lone; Vesikari, Timo et al. (2005) A hexavalent human rotavirus-bovine rotavirus (UK) reassortant vaccine designed for use in developing countries and delivered in a schedule with the potential to eliminate the risk of intussusception. J Infect Dis 192 Suppl 1:S22-9

Yuan, Lijuan; Ishida, Shin-Ichi; Honma, Shinjiro et al. (2004) Homotypic and heterotypic serum isotype-specific antibody responses to rotavirus nonstructural protein 4 and viral protein (VP) 4, VP6, and VP7 in infants who received selected live oral rotavirus vaccines. J Infect Dis 189:1833-45

Hoshino, Yasutaka; Jones, Ronald W; Ross, Jerri et al. (2004) Rotavirus serotype G9 strains belonging to VP7 gene phylogenetic sequence lineage 1 may be more suitable for serotype G9 vaccine candidates than those belonging to lineage 2 or 3. J Virol 78:7795-802