Abstract

A transient transfection system for Giardia lamblia was previously developed. Although useful for some experiments, its usefulness was limited. A stable transfection system was developed in Giardia and employed to stably express foreign proteins. The system has great utility to study cellular biology of Giardia and understand the control of variant surface protein expression.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000350-15
Application #
6160594
Study Section
Special Emphasis Panel (LPD)
Project Start
Project End
Budget Start
Budget End
Support Year
15
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Worgall, Tilla S; Davis-Hayman, Sara R; Magana, Marissa M et al. (2004) Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter: evidence for SREBP as an ancient transcription factor. J Lipid Res 45:981-8
Davis-Hayman, Sara R; Hayman, J Russell; Nash, Theodore E (2003) Encystation-specific regulation of the cyst wall protein 2 gene in Giardia lamblia by multiple cis-acting elements. Int J Parasitol 33:1005-12
Davis-Hayman, Sara R; Nash, Theodore E (2002) Genetic manipulation of Giardia lamblia. Mol Biochem Parasitol 122:1-7
Nash, Theodore E (2002) Surface antigenic variation in Giardia lamblia. Mol Microbiol 45:585-90
Hayman, J R; Hayes, S F; Amon, J et al. (2001) Developmental expression of two spore wall proteins during maturation of the microsporidian Encephalitozoon intestinalis. Infect Immun 69:7057-66
Nash, T E; Lujan, H T; Mowatt, M R et al. (2001) Variant-specific surface protein switching in Giardia lamblia. Infect Immun 69:1922-3
Elmendorf, H G; Singer, S M; Nash, T E (2001) The abundance of sterile transcripts in Giardia lamblia. Nucleic Acids Res 29:4674-83
Singer, S M; Elmendorf, H G; Conrad, J T et al. (2001) Biological selection of variant-specific surface proteins in Giardia lamblia. J Infect Dis 183:119-24
Elmendorf, H G; Singer, S M; Pierce, J et al. (2001) Initiator and upstream elements in the alpha2-tubulin promoter of Giardia lamblia. Mol Biochem Parasitol 113:157-69