Respiratory Syncytial Virus Glycoproteins

Collins, P L.

Abstract

Human respiratory syncytial (RS) virus encodes two envelope-associated glycoproteins, the fusion (F) glycoprotein and the larger (G) glycoprotein. As described previously, the complete amino sequence of the F glycoprotein was deduced by nucleotide sequence analysis of a nearly-complete cDNA of the mRNA. Recently, a complete cDNA of the mRNA encoding the G glycoprotein was isolated and analyzed by DNA sequencing. The predicted mRNA sequence encodes a protein of 298 amino acids, consistent with the estimated molecular weight of the in vitro translation product synthesized in response to hybrid-selected mRNA. Taken together with results from other laboratories, the predicted amino acid sequence shows that the G glycoprotein contains a remarkably high content of N-linked and O-linked carbohydrate and probably is anchored in the membrane by a hydrophobic domain located near the N-terminus.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000372-03
• Application #: 4688505
• Support Year: 3
• Fiscal Year: 1985

Institution

• Name: Niaid Extramural Activities
• Country: United States

Publications

Buchholz, Ursula J; Ward, Jerrold M; Lamirande, Elaine W et al. (2009) Deletion of nonstructural proteins NS1 and NS2 from pneumonia virus of mice attenuates viral replication and reduces pulmonary cytokine expression and disease. J Virol 83:1969-80

Munir, Shirin; Le Nouen, Cyril; Luongo, Cindy et al. (2008) Nonstructural proteins 1 and 2 of respiratory syncytial virus suppress maturation of human dendritic cells. J Virol 82:8780-96

Collins, Peter L; Graham, Barney S (2008) Viral and host factors in human respiratory syncytial virus pathogenesis. J Virol 82:2040-55

Chapman, Joanna; Abbott, Elizabeth; Alber, Dagmar G et al. (2007) RSV604, a novel inhibitor of respiratory syncytial virus replication. Antimicrob Agents Chemother 51:3346-53

Krempl, Christine D; Wnekowicz, Anna; Lamirande, Elaine W et al. (2007) Identification of a novel virulence factor in recombinant pneumonia virus of mice. J Virol 81:9490-501

Vallbracht, Simone; Jessen, Birthe; Mrusek, Sonja et al. (2007) Influence of a single viral epitope on T cell response and disease after infection of mice with respiratory syncytial virus. J Immunol 179:8264-73

Harker, James; Bukreyev, Alexander; Collins, Peter L et al. (2007) Virally delivered cytokines alter the immune response to future lung infections. J Virol 81:13105-11

Chi, Bo; Dickensheets, Harold L; Spann, Kirsten M et al. (2006) Alpha and lambda interferon together mediate suppression of CD4 T cells induced by respiratory syncytial virus. J Virol 80:5032-40

Bukreyev, Alexander; Serra, Maria Elina; Laham, Federico R et al. (2006) The cysteine-rich region and secreted form of the attachment G glycoprotein of respiratory syncytial virus enhance the cytotoxic T-lymphocyte response despite lacking major histocompatibility complex class I-restricted epitopes. J Virol 80:5854-61

Kotelkin, Alexander; Belyakov, Igor M; Yang, Lijuan et al. (2006) The NS2 protein of human respiratory syncytial virus suppresses the cytotoxic T-cell response as a consequence of suppressing the type I interferon response. J Virol 80:5958-67