Abstract

The focus of these studies has been on the functional role of the MHC class I molecule and the development of model systems for examining this function both in vitro and in vivo. Specific accomplishments during the past year include: 1) quantitative measurement of the interaction of MHC class I molecules-peptide complexes with T cell receptors; 2) the evaluation of the role of the MHC class I molecule H-2Dd in the signalling of a subset of NK cells bearing the cell surface marker Ly-49; 3) the quantitative evaluation and mathematical modeling of the contribution of MHC and peptide to T cell stimulation; and 4) the production of single chain MHC class I molecules for functional and binding studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000394-11
Application #
3746531
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Morozov, Giora I; Zhao, Huaying; Mage, Michael G et al. (2016) Interaction of TAPBPR, a tapasin homolog, with MHC-I molecules promotes peptide editing. Proc Natl Acad Sci U S A 113:E1006-15
Margulies, David H (2009) Home schooling of NK cells. Immunity 30:313-5
Hu, Jin-Shan; Plaksin, Daniel; Margulies, David H (2005) Backbone and side chain resonance assignmentsof a TRAV14-3 mouse T cell receptor domain. J Biomol NMR 31:271-2
Margulies, David H (2005) Monoclonal antibodies: producing magic bullets by somatic cell hybridization. J Immunol 174:2451-2
Candon, Sophie; McHugh, Rebecca S; Foucras, Gilles et al. (2004) Spontaneous organ-specific Th2-mediated autoimmunity in TCR transgenic mice. J Immunol 172:2917-24
Kuribayashi, Hideki; Wakabayashi, Ayako; Shimizu, Masumi et al. (2004) Resistance to viral infection by intraepithelial lymphocytes in HIV-1 P18-I10-specific T-cell receptor transgenic mice. Biochem Biophys Res Commun 316:356-63
Dam, Julie; Guan, Rongjin; Natarajan, Kannan et al. (2003) Variable MHC class I engagement by Ly49 natural killer cell receptors demonstrated by the crystal structure of Ly49C bound to H-2K(b). Nat Immunol 4:1213-22
Lukashev, Dmitriy E; Caldwell, Charles C; Chen, Pearl et al. (2003) A serine/threonine phosphorylation site in the ectodomain of a T cell receptor beta chain is required for activation by superantigen. J Recept Signal Transduct Res 23:33-52
Margulies, David H (2003) CD28, costimulator or agonist receptor? J Exp Med 197:949-53
Margulies, David H (2003) Molecular interactions: stiff or floppy (or somewhere in between?). Immunity 19:772-4

Showing the most recent 10 out of 34 publications