Recombinant Vaccines Against Retroviruses Associated with Leukemia and AIDS

Moss, B.

Abstract

Human immunodeficiency virus (HIV) has been identified as the etiological agent of human immunodeficiency syndrome (AIDS). At present, there is no effective vaccine against this disease. The object of this investigation is to characterize HIV antigens, determine the targets of humoral and cell mediated immunity and to use this information to develop candidate vaccines. These viruses have been used as live vaccines to immunize animals, to synthesize subunit proteins, and to make targets for cytotoxic T cells. Our finding that HIV infected individuals have circulating cytotoxic T cells directed to conserved epitopes within reverse transcriptase, as well as gag and env, may be significant for vaccine development.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000416-05
• Application #: 3818223
• Support Year: 5
• Fiscal Year: 1988

Institution

• Name: Niaid Extramural Activities
• Country: United States

Publications

Cox, Josephine H; Ferrari, Maria G; Earl, Patricia et al. (2012) Inclusion of a CRF01_AE HIV envelope protein boost with a DNA/MVA prime-boost vaccine: Impact on humoral and cellular immunogenicity and viral load reduction after SHIV-E challenge. Vaccine 30:1830-40

Wyatt, Linda S; Earl, Patricia L; Xiao, Wei et al. (2009) Elucidating and minimizing the loss by recombinant vaccinia virus of human immunodeficiency virus gene expression resulting from spontaneous mutations and positive selection. J Virol 83:7176-84

Wyatt, Linda S; Belyakov, Igor M; Earl, Patricia L et al. (2008) Enhanced cell surface expression, immunogenicity and genetic stability resulting from a spontaneous truncation of HIV Env expressed by a recombinant MVA. Virology 372:260-72

Wyatt, Linda S; Earl, Patricia L; Vogt, Jennifer et al. (2008) Correlation of immunogenicities and in vitro expression levels of recombinant modified vaccinia virus Ankara HIV vaccines. Vaccine 26:486-93

Earl, Patricia L; Americo, Jeffrey L; Wyatt, Linda S et al. (2007) Recombinant modified vaccinia virus Ankara provides durable protection against disease caused by an immunodeficiency virus as well as long-term immunity to an orthopoxvirus in a non-human primate. Virology 366:84-97

Brave, Andreas; Boberg, Andreas; Gudmundsdotter, Lindvi et al. (2007) A new multi-clade DNA prime/recombinant MVA boost vaccine induces broad and high levels of HIV-1-specific CD8(+) T-cell and humoral responses in mice. Mol Ther 15:1724-33

Lai, Lilin; Vodros, Dalma; Kozlowski, Pamela A et al. (2007) GM-CSF DNA: an adjuvant for higher avidity IgG, rectal IgA, and increased protection against the acute phase of a SHIV-89.6P challenge by a DNA/MVA immunodeficiency virus vaccine. Virology 369:153-67

Robinson, Harriet L; Sharma, Sunita; Zhao, Jun et al. (2007) Immunogenicity in macaques of the clinical product for a clade B DNA/MVA HIV vaccine: elicitation of IFN-gamma, IL-2, and TNF-alpha coproducing CD4 and CD8 T cells. AIDS Res Hum Retroviruses 23:1555-62

Liu, Jinyan; Hellerstein, Michael; McDonnel, Michael et al. (2007) Dose-response studies for the elicitation of CD8 T cells by a DNA vaccine, used alone or as the prime for a modified vaccinia Ankara boost. Vaccine 25:2951-8

Kwissa, Marcin; Amara, Rama R; Robinson, Harriet L et al. (2007) Adjuvanting a DNA vaccine with a TLR9 ligand plus Flt3 ligand results in enhanced cellular immunity against the simian immunodeficiency virus. J Exp Med 204:2733-46