Synthesis, Processing and Functions of the Proteins of Human Rsv

Collins, P L.

Abstract

We previously developed an experimental """"""""rescue"""""""" system for respiratory syncytial virus (RSV) based on a cDNA-encoded """"""""minigenome"""""""" bearing a foreign marker gene, such as that encoding chloramphenicol acetyl transferase (CAT). These minigenomes can be rendered competent for transcription, replication and incorporation into virions by complementation with proteins supplied by standard RSV helper (accompanying report). We are now attempting to modify this system such that the helper RSV is replaced by RSV proteins synthesized from vectors. This would allow us to supply the proteins in different combinations and amounts. It would then be possible to identify the viral proteins required for the major steps in the viral replicative cycle, in particular transcription, replication and virion formation. This would assign functions to the viral proteins, a number of which are completely uncharacterized. Also, the system could be used to perform detailed structure-function studies of individual proteins involved in all stages of the replicative cycle and would be the method of choice for rescuing complete infectious virus from cDNA-encoded genome RNA.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000498-08
• Application #: 3746557
• Support Year: 8
• Fiscal Year: 1994

Institution

• Name: National Institute of Allergy and Infectious Diseases
• Country: United States

Publications

McGivern, David R; Collins, Peter L; Fearns, Rachel (2005) Identification of internal sequences in the 3' leader region of human respiratory syncytial virus that enhance transcription and confer replication processivity. J Virol 79:2449-60

Zhang, Liqun; Bukreyev, Alexander; Thompson, Catherine I et al. (2005) Infection of ciliated cells by human parainfluenza virus type 3 in an in vitro model of human airway epithelium. J Virol 79:1113-24

Schomacker, Henrick; Collins, Peter L; Schmidt, Alexander C (2004) In silico identification of a putative new paramyxovirus related to the Henipavirus genus. Virology 330:178-85

Tran, Kim C; Collins, Peter L; Teng, Michael N (2004) Effects of altering the transcription termination signals of respiratory syncytial virus on viral gene expression and growth in vitro and in vivo. J Virol 78:692-9

Spann, Kirsten M; Collins, Peter L; Teng, Michael N (2003) Genetic recombination during coinfection of two mutants of human respiratory syncytial virus. J Virol 77:11201-11

Kotelkin, Alexander; Prikhod'ko, Elena A; Cohen, Jeffrey I et al. (2003) Respiratory syncytial virus infection sensitizes cells to apoptosis mediated by tumor necrosis factor-related apoptosis-inducing ligand. J Virol 77:9156-72

Zhang, Liqun; Peeples, Mark E; Boucher, Richard C et al. (2002) Respiratory syncytial virus infection of human airway epithelial cells is polarized, specific to ciliated cells, and without obvious cytopathology. J Virol 76:5654-66

Teng, Michael N; Collins, Peter L (2002) The central conserved cystine noose of the attachment G protein of human respiratory syncytial virus is not required for efficient viral infection in vitro or in vivo. J Virol 76:6164-71

Techaarpornkul, Sunee; Collins, Peter L; Peeples, Mark E (2002) Respiratory syncytial virus with the fusion protein as its only viral glycoprotein is less dependent on cellular glycosaminoglycans for attachment than complete virus. Virology 294:296-304

Gower, T L; Peeples, M E; Collins, P L et al. (2001) RhoA is activated during respiratory syncytial virus infection. Virology 283:188-96