Molecular Definition Of Filarial And Related Nonfilarial

Nutman, Thomas B.

Abstract

Using an EST (expressed sequence tag) approach, we have been able to identify greater than 965 gene clusters from Wuchereria bancrofti microfilariae based on1687 ESTs. Interestingly, a surprisingly high percentage (584/965; 60%) of these clustered ESTs contain a putative signal peptide, and only 6 appear to be GPI anchored. When compared to Brugia malayi (Bm) mf ESTs/Clusters, 45% of the Wb clusters had matches to Bm ESTs (identities ranging from 86-100%) whereas fewer (22%) matched Onchocerca volvulus (Ov) mf ESTs (with identities ranging from 80-100%). A similar approach has been taken with Strongyloides stercoralis with >10000 ESTs being done from two separate lifecylcle stages. Comparisons between this parasitic nematode and the free-living C. elegans has been perfomed.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000512-16
• Application #: 6808229
• Study Section: (HIS)
• Support Year: 16
• Fiscal Year: 2003

Institution

• Name: Niaid Extramural Activities
• Country: United States

Publications

Ghedin, Elodie; Wang, Shiliang; Spiro, David et al. (2007) Draft genome of the filarial nematode parasite Brugia malayi. Science 317:1756-60

Gomez Gallego, Sara; Loukas, Alex; Slade, Robert W et al. (2005) Identification of an astacin-like metallo-proteinase transcript from the infective larvae of Strongyloides stercoralis. Parasitol Int 54:123-33

Ravi, Varatharajalu; King, Te Piao; Andersen, John F et al. (2005) Strongyloides stercoralis recombinant NIE antigen shares epitope with recombinant Ves v 5 and Pol a 5 allergens of insects. Am J Trop Med Hyg 72:549-53

Kerepesi, Laura A; Keiser, Paul B; Nolan, Thomas J et al. (2005) DNA immunization with Na+-K+ ATPase (Sseat-6) induces protective immunity to larval Strongyloides stercoralis in mice. Infect Immun 73:2298-305

Gnanasekar, Munirathinam; Rao, Kakaturu V N; He, Yi-Xun et al. (2004) Novel phage display-based subtractive screening to identify vaccine candidates of Brugia malayi. Infect Immun 72:4707-15

Kerepesi, Laura A; Nolan, Thomas J; Schad, Gerhard A et al. (2004) Human immunoglobulin G mediates protective immunity and identifies protective antigens against larval Strongyloides stercoralis in mice. J Infect Dis 189:1282-90

Mitreva, Makedonka; McCarter, James P; Martin, John et al. (2004) Comparative genomics of gene expression in the parasitic and free-living nematodes Strongyloides stercoralis and Caenorhabditis elegans. Genome Res 14:209-20

Ravi, V; Kubofcik, J; Bandopathyaya, S et al. (2004) Wuchereria bancrofti: cloning and characterization of heat shock protein 70 from the human lymphatic filarial parasite. Exp Parasitol 106:1-10

Wu, Yang; Egerton, Gillian; McCarthy, James et al. (2003) Human immune responses to infective stage larval-specific chitinase of filarial parasite, Onchocerca volvulus, Ov-CHI-1. Filaria J 2:6

Klion, Amy D; Vijaykumar, Aarthi; Oei, Tamara et al. (2003) Serum immunoglobulin G4 antibodies to the recombinant antigen, Ll-SXP-1, are highly specific for Loa loa infection. J Infect Dis 187:128-33